Gene Silencing and Pathway Analysis

For highly flexible, economical RNAi screening
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For knockdown of human, mouse, or rat gene expression by RNA interference
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For optimized, one-step siRNA transfection
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For siRNA transfection optimization and positive control experiments in human cells
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For siRNA transfection optimization and positive control experiments in mouse or rat cells
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For negative control RNAi experiments
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For transfection of eukaryotic cells with siRNA and miRNA
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For efficient mRNA transfection
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For SYBR Green real-time one-step RT-PCR analysis from cultured cells without RNA purification
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For high-speed preparation of cDNA without RNA purification for use in real-time RT-PCR
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For multiplex, real-time one-step RT-PCR analysis from cultured cells without RNA purification
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For probe-based real-time one-step RT-PCR analysis from cultured cells without RNA purification
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For purification of microRNA and total RNA from tissues and cells
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For simultaneous purification of DNA, RNA, and protein from cells and tissues
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For simultaneous purification of DNA and RNA from cells and tissues
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For fully automated sample prep using QIAGEN spin-column kits
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For fully integrated automation of complete workflows, from sample preparation to assay setup
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For fully automated medium- to high-throughput applications in 96-well format
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For SYBR® Green-based, real-time PCR profiling of miRNAs using the miScript PCR System
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For preamplification of cDNA from samples containing low RNA amounts prior to real-time PCR analysis of miRNA
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For miRNA detection by real-time PCR using the miScript PCR System
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For quantification of mature miRNA using the miScript PCR System
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For reverse transcription of total RNA containing miRNA using the miScript PCR System
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For discovery of signaling pathway response to gene modification or chemical treatment
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For rapid, sensitive, and quantitative assessment of signal transduction pathway activation
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For control experiments when using Cignal Reporter Assays
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For sensitive assessment of cell signaling activities in virtually any mammalian cell type
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For control experiments when using Cignal Lenti Reporter Assays
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For sensitive assessment of cell signaling activities in virtually any mammalian cell type
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For discovery of signaling pathway response to gene modification or chemical treatment
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For use with the miScript PreAMP PCR Kit
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