text.skipToContent text.skipToNavigation

PAXgene Tissue STABILIZER

For stabilization of tissue specimens previously fixed in PAXgene Tissue FIX
  • Preservation of both morphology and biomolecules
  • Tissue can be stored and archived for later processing
  • RNA, miRNA, DNA, and/or proteins from one sample
PAXgene Tissue STABILIZER is used for transport or storage of tissue specimens fixed with the PAXgene Tissue FIX Container (50 ml). After fixation of tissue samples with the PAXgene Tissue FIX Container (50 ml), PAXgene Tissue FIX is removed and replaced by PAXgene Tissue STABILIZER within the same container. Nucleic acids, proteins and morphology of the sample are now stable up to 7 days at room temperature, or for longer periods at 2–8°C, or even at –20°C and –80°C. Stabilized samples can be embedded in paraffin for histological studies. PAXgene Tissue Kits and supplementary protocols provide efficient subsequent purification of RNA, miRNA, DNA, and/or proteins from the same sample.

PAXgene Tissue STABILIZER Concentrate is only to be used in conjunction with tissue specimens previously fixed in PAXgene Tissue FIX.

Buy Products

Cat No./ID: 765512
PAXgene Tissue STABILIZER Concentrate (150 ml)
€185.00
Order Product
8 bottles of PAXgene Tissue Stabilizer concentrate, for 4 liters of PAXgene Tissue STABILIZER
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.

Product Details

0
Preservation of tissue morphology.
Tissue was fixed and stabilized with the PAXgene Tissue FIX Container (50 ml); 4 µm sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue were stained with hematoxylin and eosin (HE). [A] Rat liver, [B] rat kidney, [C] rat intestine. Overview at 40x, details at 400x magnification.
0
Yield of high-quality, high molecular weight DNA from 12 different PAXgene Tissue-fixed (PF) tissue types, processed on the QIAcube.

Twelve different PAXgene Tissue-fixed (PF) rat tissue types, including fibrous or fatty, DNA-rich and DNA-poor tissues, processed with the PAXgene Tissue DNA Kit on the QIAcube in one single run. DNA yield per 10 mg tissue.

1
Absorbance ratio of high-quality, high molecular weight DNA from 12 different PAXgene Tissue-fixed, paraffin-embedded (PF) tissue types, processed on the QIAcube.

Twelve different PAXgene Tissue-fixed (PF) rat tissue types, including fibrous or fatty, DNA-rich and DNA-poor tissue, processed with the PAXgene Tissue DNA Kit on the QIAcube in a single run. Ratio of absorbance at 260 and 280 nm.

2
Comparison of manual and automated procedure: DNA yield from sections of PAXgene Tissue-fixed, paraffin-embedded (PFPE) tissue.

DNA yield from 3 sections (thickness 10 µm) each of 8 different PAXgene Tissue-fixed, paraffin-embedded (PFPE) rat tissue types processed in triplicate with the PAXgene Tissue DNA Kit on the QIAcube or manually.

3
Comparison of manual and automated procedure: Absorbance ratio from sections of PAXgene Tissue-fixed, paraffin-embedded (PFPE) tissue.

Ratio of absorbance at 260 and 280 nm from 3 sections (thickness 10 µm) each of 8 different PAXgene Tissue-fixed, paraffin-embedded (PFPE) rat tissue types processed in triplicate with the PAXgene Tissue DNA Kit on the QIAcube or manually.

4
DNA without chemical modification can be used for demanding downstream applications.

Multiplex and long-range PCR of DNA from PAXgene Tissue-fixed, paraffin-embedded (PFPE) human colorectal cancer tissue (modified according to Viertler et al., (2012) A new technology for stabilization of biomolecules in tissues for combined histological and molecular analyses. J. MOl. Diagn. 14, 458). [A] Multiplex PCR of 8 different genomic DNA fragments ranging from 22 to 955 bp. [B] Long-range PCR of a 5 kb genomic DNA fragment.

5
Protein extracted from PFPE tissue is suitable for two-dimentional gel electrophoresis.

Non-malignant human duodenum tissue was divided into 3 samples and either flash-frozen in liquid nitrogen (cryo), or prepared as PFPE or FFPE tissue. Proteins from cryo and PFPE tissue were extracted in 2D buffer (30 mM Tris-HCl, pH 8.8, 7 M urea, 2 M thiourea, 4% CHAPS, 75 mM DTT) supplemented with protease inhibitor. Proteins from FFPE tissue were extracted in EXB Plus buffer supplemented with protease inhibitor, precipitated with acetone and resuspended in 2D buffer (as described in Guendisch et al. 2013). Samples (150 µg) were separated by 2-D PAGE. Data kindly provided by Karl-Friedrich Becker, Technical University of Munich, Germany.

6
Agarose gel electrophoresis of high-quality, high molecular weight DNA from 12 different PAXgene Tissue-fixed (PF) tissue types, processed on the QIAcube.

Twelve different PAXgene Tissue-fixed (PF) rat tissue types, including fibrous or fatty, DNA-rich and DNA-poor tissue, processed with the PAXgene Tissue DNA Kit on the QIAcube in one single run. DNA from each tissue type (300 ng) on agarose gel electrophoresis using 1% TBE buffered gels; M: markers.

7
Comparison of manual and automated procedure: Agarose gel electrophoresis from sections of PAXgene Tissue-fixed, paraffin-embedded (PFPE) tissue.

Agarose gel electrophoresis from 3 sections (thickness 10 µm) each of 8 different PAXgene Tissue-fixed, paraffin-embedded (PFPE) rat tissue types processed in triplicate with the PAXgene Tissue DNA Kit on the QIAcube and manually. DNA from each replicate and tissue type (200 ng) on agarose gel electrophoresis using 1% TBE buffered gels; M: markers.

8
Detection of nondegraded, immunoreactive phosphoproteins from human clinical PFPE tissue.

Non-malignant human uterus, breast, prostate and bladder tissue specimens were divided into 3 samples and either flash-frozen in liquid nitrogen (cryo), PAXgene Tissue-fixed, paraffin-embedded (PFPE) or formalin-fixed, paraffin-embedded (FFPE). Proteins from cryo, PFPE and FFPE tissues were extracted using the extraction buffer EXB Plus (Qproteome FFPE Tissue Kit; described in Ergin et al. 2010, Guendisch et al. 2013 and PAXgene Tissue supplementary protocols). SDS-PAGE and Western blot analysis were performed with 15 µg protein and the indicated antibodies. Data kindly provided by Karl-Friedrich Becker, Technical University of Munich, Germany.

9
High concordance of miRNA expression between total RNA isolated from PFPE and fresh-frozen tissue.

RNA, including miRNA, was purified from mirrored human breast cancer tissue fresh frozen in liquid nitrogen using the QIAGEN miRNeasy Kit, or from sections of PAXgene Tissue-fixed, paraffin-embedded (PFPE) tissue using the PAXgene Tissue RNA/miRNA Kit. Shown is a scatterplot of CT values from different single miRNA-specific RT-qPCR assays using the QIAGEN miScript System: miR9, -10a, -10b, -29a, -103, -125b, -143, -145, -192 and miScript PCR controls RNUA1, RNU5A, RNU6B, SNORD25, SCARNA19, SNORA73A; R2: coefficient of determination.

10
RNA purified without chemical modification from PFPE tissue using the PAXgene Tissue RNA/miRNA Kit.

SYBR Green real-time RT-qPCR was performed with 10 ng RNA from cryopreserved, formalin-fixed, paraffin-embedded (PPFE) or PAXgene Tissue-fixed, paraffin-embedded (PFPE) rat tissue (modified according to Groelz et al. 2013). Depicted are the average delta-CT values (delta-CT = CT[FFPE] – CT[cryo] or delta-CT = CT[PFPE] – CT[cryo]) from 6 different assays with amplicons ranging from 109 to 465 bp.

11
Immunohistochemistry (IHC) staining with the PAXgene Tissue System gives comparable results to formalin-fixed tissue.

Human palatine tonsil tissue was fixed in PAXgene Tissue FIX or with neutral-buffered formalin and embedded in paraffin. Primary antibodies to the indicated antigens were linked to a streptavidin-peroxidase conjugate by a biotinylated secondary antibody (LSAB method). Sectiosn were counterstained with hematoxylin. PFPE: PAXgene Tissue-fixed, paraffin-embedded. FFPE: formalin-fixed, paraffin-embedded.

12
H&E staining with the PAXgene Tissue System gives results comparable to formalin-fixed tissue.

Human tissue samples were divided into 2 sub-samples. One sub-sample was fixed with PAXgene Tissue FIX and the other was fixed with neutral-buffered formalin. The fixed tissues were embedded in paraffin, sectioned and stained with hematoxylin and eosin. PFPE: PAXgene Tissue-fixed, paraffin-embedded. FFPE: formalin-fixed, paraffin-embedded.

13
The PAXgene Tissue FIX Container (50 ml) and PAXgene Tissue STABILIZER workflow.

Single-chamber container for fixation and stabilization of a single, larger or multiple, smaller tissue samples.

14
High-quality DNA from PFPE tissue with preserved morphology.

[A] Hematoxylin and eosin (H&E) staining of PAXgene Tissue-fixed, paraffin-embedded (PFPE) human colorectal cancer tissue and [B] DNA on agarose gel electrophoresis using 0.8% TBE buffered gels with 200 ng genomic DNA isolated in triplicate from 5 cases (#1–5) of human PFPE colorectral cancer. M: markers.

Performance
The fixation and stabilization method preserves tissue morphology and the integrity of nucleic acids without destructive crosslinking and degradation found in formalin-fixed tissues (see figure "Preservation of tissue morphology").

When fixed tissue is stored in PAXgene Tissue STABILIZER, the nucleic acids, proteins, and morphology of the tissue sample are stable for up to 7 days at room temperature (15–25°C) or for up to 4 weeks at 2–8°C. Tissue samples can even be stored in the PAXgene Tissue STABILIZER for longer periods at –20°C (–15°C to –30°C) or –80°C (–65°C to –90°C) without negative effects on the morphology of the tissue or the integrity of the nucleic acids.

Specifications for storage conditions in PAXgene Tissue STABILIZER were determined using animal tissues.
Principle

The methods for tissue fixation currently used in traditional histology are of limited use for molecular analysis. Fixatives that contain formaldehyde crosslink biomolecules and modify nucleic acids and proteins. Such crosslinks lead to nucleic acid degradation during tissue fixation, storage, and processing. Since they cannot be removed completely, the resulting chemical modifications can cause inhibition in downstream applications, such as quantitative PCR or RT-PCR. To enable both molecular and traditional pathology testing from the same specimen, a method is needed to stabilize molecular content and preserve morphology.

To meet this need, PreAnalytiX has developed the PAXgene Tissue System. The system consists of a fixation reagent (PAXgene Tissue FIX), a stabilization reagent (PAXgene Tissue STABILIZER), prefilled containers for tissue collection, storage, and transportation, and kits for purification of RNA, DNA, or total RNA, including miRNA. In addition, supplementary protocols for protein purification and other applications are available in the 'Resources' section of this page or at www.preanalytix.com.

PAXgene Tissue reagents in prefilled containers and PAXgene Tissue Kits provide a complete preanalytical solution for collection, fixation, and stabilization of tissue, and purification of high-quality nucleic acids for molecular research analysis.

Procedure
After fixation of tissue samples with the PAXgene Tissue FIX Container (50 ml), the fixative in the container is removed and replaced by PAXgene Tissue STABILIZER within the same container. Stabilized samples can be embedded in paraffin for histological studies. Nucleic acids and proteins can be isolated from the stabilized samples before or after embedding in paraffin. See the PAXgene Tissue Kit Handbooks for information about DNA, RNA, or miRNA, and the PAXgene Tissue supplementary protocols in the 'Resources' section of this page or at www.preanalytix.com for protein purification and other applications.
Applications
Sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue can be used for histological studies or extraction of nucleic acids or proteins. Purification of RNA, total RNA, including miRNA, or DNA from PAXgene Tissue fixed and stabilized tissue samples requires the use of one of the PAXgene Tissue Kits for RNA, miRNA, or DNA. Purification of protein requires the Qproteome FFPE Kit.

Product Resources

You are not authorized to download the resource

Brochures & Guides (5)
For isolation and purification of genomic DNA from tissue samples stabilized using the PAXgene Tissue System 

 


Show details

 


Show details
For isolation and purification of intracellular RNA from tissue samples stabilized using the PAXgene Tissue System

 


Show details
For extraction of full-length proteins from PFPE tissue using the Qproteome FFPE Tissue Kit

 


Show details
Moving toward excellence and standardization in tissue collection and fixation

 


Show details
FAQs (34)
FAQ ID -2518
View
FAQ ID -2519
View
FAQ ID -2524
View
FAQ ID -2525
View
FAQ ID -2526
View
FAQ ID -2529
View
FAQ ID -2530
View
FAQ ID -2531
View
FAQ ID -2533
View
FAQ ID -2534
View
FAQ ID -2538
View
FAQ ID - 3513
View
FAQ ID - 3600
View
FAQ ID - 3601
View
FAQ ID - 3602
View
FAQ ID - 3603
View
FAQ ID - 3604
View
FAQ ID - 3605
View
FAQ ID - 3606
View
FAQ ID - 3607
View
FAQ ID - 3608
View
FAQ ID - 3609
View
FAQ ID - 3610
View
FAQ ID - 3611
View
FAQ ID - 3612
View
FAQ ID - 3613
View
FAQ ID - 3614
View
FAQ ID - 3615
View
FAQ ID - 3616
View
FAQ ID - 3617
View
FAQ ID - 3618
View
FAQ ID - 3619
View
FAQ ID -3030
View
FAQ ID -3032
View
Scientific Posters (14)
Long et al., ADAPT 2012

 


Show details
Hesse et al., AACR-NCI-EORTC 2011

 


Show details
Groelz et al., AMP 2010 

 


Show details
Groelz et al., ECP 2012

 


Show details
Groelz et al., 3rd Annual Oncology Biomarkers 2011

 


Show details
Groelz et al., BRN Symposium 2011 

 


Show details
Groelz et al., AMP 2014

 


Show details
Groelz et al., ISBER 2012

 


Show details
Groelz et al., AACR 2010

 


Show details
Groelz et al., BRN Symposium 2012 

 


Show details
Groelz et al., AMP 2009 

 


Show details
Groelz et al., ECP 2014

 


Show details
Groelz et al., AMP 2008 

 


Show details
Groelz et al., AMP 2009

 


Show details
Supplementary Protocols (16)

Show details

Show details

Show details

Show details

Show details

Show details

Show details

Show details

Show details

Show details

Show details

Show details

Show details

Show details

Show details

Show details
Kit Handbooks (2)
For collection, fixation, and stabilization of multiple small tissue samples or a single large tissue sample

 


Show details
For stabilization of tissue specimens fixed in PAXgene Tissue FIX Container

 


Show details
References
0
fragment fix placeholder

Customers who bought these products also bought

  • Cat No./ID: 765312
    /at/products//discovery-and-translational-research/sample-collection-stabilization/tissue-ffpe/paxgene-tissue-fix-container-50-ml/

    PAXgene Tissue FIX Container (50 ml)

    For fixation and stabilization of tissue specimen: 10 prefilled Reagent Containers containing 50 ml of PAXgene Tissue FIX
    €133.00
  • Cat No./ID: 766134
    /at/products//discovery-and-translational-research/sample-collection-stabilization/rna/paxgene-tissue-rna-mirna-kit/

    PAXgene Tissue RNA/miRNA Kit (50)

    For 50 RNA preps: PAXgene RNA MinElute Spin Columns, PAXgene Shredder Spin Columns, Processing Tubes, Microcentrifuge Tubes, Carrier RNA, RNase-Free DNase, and RNase-Free Buffers; to be used in conjunction with PAXgene Tissue Containers
    €478.00