New technology and workflow for integrated collection, stabilization and purification of circulating cell-free DNA
PreAnalytiX, a QIAGEN joined venture with BD, has developed the PAXgene Blood ccfDNA System, consisting of the PAXgene Blood ccfDNA Tube, and the QIAsymphony PAXgene Blood ccfDNA Kit for automated ccfDNA extraction from up to 5 ml of plasma. The slidedeck presents this new technology development and a streamlined workflow for integrated collection, stabilization and purification of circulating cell-free DNA from whole blood.
Automate circulating cell-free DNA isolation using the QIAsymphony
Looking for an automated and standardized process to isolate circulating cell-free DNA? This slidedeck features an optimized process for efficient cfDNA enrichment and extraction from plasma samples, and presents a novel automated solution for the extraction of cfDNA using the QIAsymphony SP instrument for liquid biopsy diagnostic applications.
New and highly integrated tools for sensitive next-generation sequencing: Analysis of circulating cell-free DNA
Frustrated with the poor quality of circulating cell-free DNA (cfDNA) isolated from blood samples? This slidedeck introduces new technical solutions for Ion Torrent, as well as Illumina sequencing platforms that combine optimized cfDNA isolation, highly advanced library construction, unbiased library amplification and integrated data analysis to reliably analyze cfDNA samples with high sensitivity.
This presentation shows how mutations can be best identified from this type of data and how they can be interpreted. Furthermore, potential challenges when analyzing this type of data will be discussed together with relevant strategies.
CTC detection and molecular characterization – challenges and solutions
Circulating tumor cells (CTCs) are rare and highly heterogeneous. In addition, during the epithelial-mesenchymal transition (EMT) process, CTCs lose their epithelial characteristics and acquire more mesenchymal-like phenotypes, which pose another challenge for CTC detection and characterization. In this slidedeck, Dr. Siegfried Hauch introduces the AdnaTests, which use the Combination of Combinations Principle (COCP) to enable enriching and detecting CTCs in whole blood with high specificity and sensitivity.
The presence and persistence of resistant and stem cell-like tumor cells is a major problem in ovarian cancer
Epithelial ovarian cancer ranks as the second most common type of gynecological malignancy and has poor survival rates. In this presentation, Dr. Sabine Kasimir-Bauer from University of Duisburg-Essen demonstrates that the negative prognostic impact of CTCs and DTCs arises from specific cellular phenotypes and is associated with platinum-based resistance and stem cell-associated proteins.