Ni-NTA Superflow

For purification of 6xHis-tagged proteins by FPLC

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Specifications
Product Resources

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Cat No./ID: 30410

Ni-NTA Superflow (25 ml)

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25 ml nickel-charged resin (max. pressure: 140 psi)

Cat No./ID: 30430

Ni-NTA Superflow (100 ml)

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100 ml nickel-charged resin (max. pressure: 140 psi)

Cat No./ID: 30450

Ni-NTA Superflow (500 ml)

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500 ml nickel-charged resin (max. pressure: 140 psi)

The Ni-NTA Superflow is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Specifications

Features	Specifications
Applications	Proteomics
Bead size	60–160 µm
Binding capacity	Up to 50 mg/ml
FPLC	Yes
Gravity flow or spin column	Gravity flow or automated
Number of preps per run	1–24 samples per run
Processing	Manual
Scale	Large scale
Special feature	Batch and column purification
Start material	Cell lysate
Support/matrix	Superflow
Tag	6xHis tag
Yield	Depends on binding capacity

Product Resources

FAQs (16)

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	What are the compatibilities of different reagents with Ni-NTA matrices? FAQ ID -49	View
	How can I remove imidazole from a protein sample? FAQ ID -91	View
	How can I eliminate contaminating protein in my Ni-NTA 6xHis-tag protein purification? FAQ ID -102	View
	Should I use Ni-NTA Agarose in column or batch format for purification of 6xHis-tagged proteins? FAQ ID -147	View
	What are the features and benefits of the QIAexpress 6xHis Tag System? FAQ ID -193	View
	What are your recommendations for PCR template preparation for use with the EasyXpress Insect Kit II? FAQ ID -1221	View
	How can I check if any residual proteins remain on the Ni-NTA Agarose matrix after elution? FAQ ID -324	View
	Can Ni-NTA resins be used to purify protein with an internal His-tag? FAQ ID -496	View
	Is it possible to isolate both RNA and recombinant 6xHis-tagged protein from the same sample? FAQ ID -532	View
	Why do you recommend using Triton X for the purification of 6xHis-tagged protein? -100	View
	What is the difference between Ni-NTA Agarose and Ni-NTA Superflow? FAQ ID -764	View
	How can I be sure that I am harvesting my induced bacterial culture at the best time point for protein expression? FAQ ID -788	View
	Are the buffers in the Ni-NTA Fast Start Kit the same as the ones for use with Ni-NTA purchased separately? FAQ ID -791	View
	Can I reuse the Ni-NTA Agarose and Ni-NTA Superflow resins? FAQ ID -802	View
	Which resin is used in the QIAexpress Ni-NTA Fast Start Columns? FAQ ID -836	View
	Do you have a protocol for manual purification of 6xHis-tagged proteins expressed in E. coli using Ni-NTA Superflow? FAQ ID -967	View

Kit Handbooks (2)

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	The QIAexpressionist - (EN) A handbook for high-level expression and purification of 6xHis-tagged proteins	Show details
	(EN) - Important Note for Ni-NTA Users	Show details

Technical Information (3)

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	Complete solutions for membrane protein analysis - (EN)	Show details
	Critical factors for successful protein crystallization - (EN)	Show details
	Reliable purification of GST-, His-, and Strep-tagged proteins - (EN)	Show details

Scientific Posters (3)

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	(EN) - New Ni-NTA Cartridges — the faster way to purer proteins	Show details
	(EN) - Novel cell-free expression system for synthesis of proteins used in structural analyses	Show details
	(EN) - Process Development: Scaling Up Human IL-1b Production, Tag Removal, and X-Ray Crystallography	Show details

Quick-Start Protocols (2)

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	Automated Purification of 6xHis-tagged Proteins from E. coli Using Ni-NTA Superflow under Native Conditions (EN)	Show details
	Ni-NTA Agarose Purification of 6xHis-tagged Proteins from E. coli under Native Conditions (EN)	Show details

Safety Data Sheets (4)

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	MSDS Ni-NTA Superflow (100 ml)
	MSDS Ni-NTA Superflow (25 ml)
	MSDS Ni-NTA Superflow (500 ml)
	CofA

References