The precise quantification of phytoplasmas in plants is imperative for the diagnosis and control of plant diseases. Digital PCR (dPCR) is a revolutionary technique that enables the absolute quantification of obligate biotrophic bacteria without the necessity of a calibration curve, thereby ensuring superior accuracy compared to conventional quantitative PCR (qPCR). This webinar will focus on the quantification of two significant pathogens of sugar beets: Candidatus Arsenophonus phytopathogenicus and Candidatus Phytoplasma solani. The findings of the analyses conducted indicate that dPCR is the optimal method for enhancing the diagnosis and monitoring of phytoplasma-related plant diseases. Consequently, dPCR can facilitate the optimization of plant protection measures.