PCR/qPCR/dPCR
特異的かつ高感度、堅牢なPCRを確実に
遺伝子増幅を行う際に、実験の成功はスループット、アッセイ感度、テンプレートの難易度に起因しない反応のロバストネス、データ正規化の信頼性など、いくつかの重要な要素に左右される可能性があります。使用するPCRメソッドに関連する特定の課題を克服することが必要になる場合もあります。
当社は、お客様がどのメソッドを選択しようと、エンドポイントPCR、qPCR、dPCR用に最適化された豊富なソリューションを提供します。当社のキット、アッセイ、装置、および付属品は、お客様がターゲットを確実に増幅および検出し、ほぼすべてのアプリケーションのワークフローを合理化するのに役立ちます。
- Measure gene expression (mRNA, miRNA)
- Quantify DNA or RNA targets
- Detect & quantify pathogen and/or viral RNA/DNA
- Quantify residual DNA
- Convert RNA to cDNA for quantification
- Mutation detection (including rare/ultra-rare variants)
- Analyze copy number variation (CNV)
- Detect viral load
- Gene expression quantification
- Quantify low-abundance targets
- Measure residual DNA
- Amplify DNA for cloning or sequencing
- Qualitative pathogen detection (presence/absence)
- CRISPR screening
- Genotyping
- Convert RNA to cDNA for storage
- Cq/Ct values
- Relative quantification (ΔΔCq) or absolute quantification from standards
- cDNA for downstream use
- Absolute target quantification (copies/µL reaction)
- Fractional abundance (%)
- Mutation/target frequency
- Copy number per genome
- Amplified DNA or cDNA product
- Band visualization on gel
- Presence/absence result
- Isolated DNA or cDNA
- Isolated RNA/mRNA/miRNA
- Some kits support crude samples such as cells, blood, dried blood spots, saliva, buccal swabs, or gargle
- Isolated DNA or cDNA
- Isolated RNA/mRNA/miRNA
- Purified samples recommended
- Isolated DNA or cDNA
- Isolated RNA
- Quantitative real-time PCR
- Reverse transcription
- Amplification and fluorescence-based detection in real-time for quantification
- Quantitative digital PCR
- Partitions the sample into thousands of reactions and uses end-point PCR + Poisson statistics for absolute quantification without a standard curve
- Standard end-point PCR
- DNA amplification and visualization (e.g., gel-based) and downstream applications
- When reliable quantification is required over a wide linear range
- When standard curve-based or relative quantification is sufficient
- When precise quantification is required
- When detecting rare alleles or small fold changes
- When standard curves are undesirable
- When working near the detection limit (low-abundance targets)
- When qualitative detection without quantification is sufficient
- When preparing amplicons for cloning, genotyping, sequencing, or other downstream applications
- Real-time PCR thermal cycler
- Dye-based (e.g., SYBR Green) or probe-based detection
- QIAcuity dPCR platform
- Probe‑based or intercalating dye detection
- Thermal cycler
- Visualization (e.g., via agarose gel electrophoresis)
閲覧 PCR/qPCR/dPCR
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5個の製品が見つかりました
QIAcuity Digital PCR System
ナノプレートを使用するデジタルPCRアプリケーション向け
dPCR Microbial DNA Detection Assay
細菌、真菌、寄生虫、ウイルス、抗生物質耐性または毒性因子遺伝子など、微生物ターゲットのデジタルPCR検出用
mericon Ingredient Authentication Assay
For real-time PCR authentication of food and animal feed ingredients
Viral Vector Direct Lysis Kits
For the direct lysis of viral vectors, such as different adeno-associated virus (AAV) serotypes and adenoviruses and lentivirus (LV)
QIAprep& Viral RNA UM Kit
For rapid and high-frequency detection of SARS-CoV-2 by quantitative real-time PCR for epidemiological research
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