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Cat. No. / ID: 873001
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
The IDH1/2 RGQ PCR Kit provides reagents to perform 9 separate amplification reactions for detection of 12 mutations:
Gene | Mutation | Base change | Cosmic ID |
---|---|---|---|
IDH1 | Arg132His | 395G>A | COSM28746 |
Arg132Cys | 394C>T | COSM28747 | |
Arg132Ser | 394C>A | COSM28748 | |
Arg132Gly | 394 C>G | COSM28749 | |
Arg132Leu | 395G>T | COSM28750 | |
Arg132Val | 394_395 CG>GT | COSM28751 | |
Arg100Gln | 299G>A | COSM88208 | |
IDH2 | Arg172Lys | 515G>A | COSM33733 |
Arg172Met | 515G>T | COSM33732 | |
Arg172Trp | 514A>T | COSM34039 | |
Arg172Ser | 516G>T | COSM34090 | |
Arg172Gly | 514A>G | COSM33731 |
The Total Primers and Probe Mixes (PPM-Total) use primers and probes to amplify both mutated and wild-type target sequences.
The mutation detection primers and probe mixes combine primers and probes, to amplify both mutated and wild-type target sequences, plus an oligonucleotide, 3' blocked with the addition of a phosphate group to prevent elongation (PCR clamping), which is specific to the wild-type target sequence.
When the PCR template contains the wild-type sequence, the 3'-phosphate oligonucleotide will dominate over PCR primer binding due to higher affinity. There is no or low extension by the DNA polymerase and no or low amplification is observed.
When a mutated sequence is present, PCR primer binding will dominate over the 3'-phosphate oligonucleotide binding and amplification will proceed.
Allele-specific amplification is achieved by ARMS (Amplification Refractory Mutation System), which exploits the ability of the DNA polymerase to distinguish between a match and a mismatch at the 3' end of a PCR primer.
When the PCR primer is fully matched, the amplification proceeds with full efficiency. When the 3' base is mismatched, only low-level background amplification occurs.