QIAsymphony Certal Kits

For purification of residual host cell DNA and viral nucleic acids from bioprocess samples

S_1084_5_GEN_V2

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QIAsymphony Certal Vaccine Kit (96)

Cat. No. / ID:  931955

For 96 preps of 1000 µl each: includes 2 reagent cartridges and enzyme racks and accessories
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Kit
QIAsymphony Certal Vaccine Kit
QIAsymphony Certal Residual DNA Kit
QIAsymphony Certal Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • Flexible and fully automated purification on the QIAsymphony SP
  • High recovery from manifold sample matrices without pre-adjustment
  • Optimized protocols for streamlined workflow and high-quality results

Product Details

The QIAsymphony Certal Residual DNA Kit and the QIAsymphony Certal Vaccine NA Kit provide automated solutions for purification of residual host cell DNA and viral nucleic acids from bioprocess purification buffer, cell culture supernatant samples, and vaccine preparations using the QIAsymphony SP. Optimized protocols enable a streamlined workflow and high-quality results. Novel, prefilled reagent cartridges reduce setup time and minimize manual handling. Bar code reading enables full tracking of reagents.

Principle

QIAsymphony Certal Kits, in combination with the QIAsymphony SP, enable fully automated purification of nucleic acids from samples generated during production and purification of cell-culture-based biotechnology-derived products, biopharmaceutical protein drugs (e.g., monoclonal antibodies), or from virus-propagating cell lines for vaccine production. Samples include cell-culture supernatants or fermentation broths after removal of cells, bioprocess purification buffers from chromatography purification procedures, or fractions from vaccine purification (e.g., after inactivation).

The QIAsymphony Certal Residual DNA Kit enables extraction of residual host cell DNA and viral nucleic acids from biologics (e.g., monoclonal antibodies) purified using chromatography procedures. A proprietary buffer (buffer CA) is used to equilibrate matrix effects caused by chromatography reagents, thereby improving the recovery of the nucleic acids from bioprocess samples.

The QIAsymphony Certal Vaccine NA Kit enables extraction of viral nucleic acids from cell-based vaccine production. A proprietary buffer (buffer CB) is used to equilibrate for matrix effects caused by chemical or physical treatment during inactivation and purification of the propagated virus from vaccine production.

Proven, performance-leading magnetic particle technology enables purification of high-quality nucleic acids that are free of proteins, nucleases, and other impurities. The purified nucleic acids are ready for direct use in downstream applications, such as amplification or other detection reactions (e.g., Threshold assay). The QIAsymphony SP performs all steps of the purification procedure. Up to 96 samples, in batches of up to 24, are processed in a single run.

For downstream detection, the QIAsymphony Certal Kits are compatible with the Certal CHO Detection Kit and the Certal Vero Detection Kit, enabling highly sensitive detection of residual genomic DNA from CHO and Vero cell lines using quantitative PCR. For high-precision results, we recommend using the Rotor-Gene Q real-time PCR cycler.

Procedure

QIAsymphony technology combines the speed and efficiency of silica-based nucleic acid purification with the convenient handling of magnetic particles. The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples, and comprises 4 steps: lyse, bind, wash, and elute. The user can choose between different eluation volumes (60 μl, 85 μl, and 110 μl), depending on the protocol (see table).

Protocols for use with QIAsymphony Certals Kits
Protocol Kit Sample material Processed volume
resDNA1000 QIAsymphony Certal Residual DNA Kit Biologics purified using
chromatography procedures
500 µl
vacNA1000 QIAsymphony Certal Vaccine NA Kit Vaccine samples; cell culture
supernatants or fermentation broths
500 µl

Worktable setup is rapid, which saves your valuable time. Simply place up to 2 reagent cartridges in the consumables drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes appropriate for the selected number of samples are used, giving you complete cost control.

No pre-conditioning with either buffer CA or buffer CB is necessary for the extraction of host-cell DNA or viral nucleic acids from cell-culture supernatant or fermentation broth.

Applications

QIAsymphony Certal Kits, in combination with the QIAsymphony SP, enable purification of residual host cell DNA and viral nucleic acids from bioprocess purification buffer, cell culture supernatant samples, and vaccine preparations.

Resources

Labware Files and Documents (1)
For use with software version 5.0 or higher 
Kit Handbooks (1)
Application/Protocol Documents (2)
vacNA1000_V3 protocol
PDF (113KB)
For use with software version 4.0 or higher
For use with software version 4.0 or higher
Certificates of Analysis (1)