Design your own 96- or 384-well miRNA qPCR plates based on a fully flexible layout and several convenient, predefined layouts. Place predesigned miRCURY LNA miRNA PCR Assays or your own Custom miRCURY PCR Assays into the panel using an intuitive and easy-to-use online plate configurator. The panels are delivered ready-to-use, with one single assay per well for a 10 µl reaction volume. Just add cDNA and miRCURY LNA SYBR Green Master Mix.
Custom miRNA PCR Panels are available in 2 different formats:
- 96-well, ready-to-use: design your panels from one of three available layouts. Choose from our vast selection of experimentally validated LNA miRNA PCR primer sets and reference gene primer sets for human, mouse and rat miRNA quantification.
- 384-well, ready-to-use: design your panels from one of four available layouts. Choose from our vast selection of experimentally validated LNA miRNA PCR primer sets and reference gene primer sets for human, mouse and rat miRNA quantification.
Custom miRNA PCR Panels come with a pre-positioned interplate calibrator (IPC) in replicates that allow optimal calibration and control assessment in multi-plate studies. The IPC is mandatory but can be re-positioned to suit your preferred plate layout. In additional, the panel is displayed with one or more pre-positioned UniSp6 RNA Spike-in controls (CP). RNA spike-in controls may be used for monitoring the cDNA synthesis or RNA purification. The UniSp6 CP assay can be removed and substituted by miRNA primer set(s) or other RNA spike-in control assays. Alternative RNA spike-in controls include UniSp2, UniSp4, UniSp5 and cel-miR-39-3p.
Add custom assays
It is now possible to add custom assays to your custom plate. Custom assays are designed through one of our custom assay design tools. Design LNA-enhanced PCR primers for sequences not available among our predesigned assays, e.g., for novel miRNAs identified using next-generation sequencing.