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SuperFect Transfection Reagent

For transfection of a broad range of eukaryotic cell lines with DNA
  • Suitable for a broad range of cell lines
  • Transfection can be performed in the presence of serum
  • Activated dendrimers ensure reproducibility

SuperFect Transfection Reagent is based on activated dendrimer technology developed for DNA transfection into a broad range of cell lines.

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Cat No./ID: 301305
SuperFect Transfection Reagent (1.2 ml)
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For 40 transfections in 60 mm dishes or 160 transfections in 12-well plates
Cat No./ID: 301307
SuperFect Transfection Reagent (4 x 1.2ml)
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For 160 transfections in 60 mm dishes or 640 transfections in 12-well plates
The SuperFect Transfection Reagent is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

0
Serum and DNA quantity vs. transfection efficiency.
The influence of serum and DNA quantity on transfection using SuperFect Reagent was examined. COS-7 cells (2 x 104 per well) were seeded in 96-well plates one day prior to transfection. Cells were transfected using 0.1–2.0 µg beta-galactosidase-reporter plasmid and 3 µl SuperFect Reagent per well, in either the presence or absence of serum. Each bar represents the average efficiency from 4 replicates assayed 48 hours after transfection.
3
Transfection of neuronal PC-12 cells using SuperFect Reagent.
Expression of green fluorescent protein (GFP) in differentiated PC-12 cells was examined 5 days post-transfection. Cells (104–105)  previously stimulated with 50 ng/ml NGF were plated per 60 mm dish one day prior to transfection. Transient transfections were performed in 2 ml low-serum growth medium (DMEM plus 0.05% FBS) using 3 µg of a GFP-reporter plasmid and 15 µl SuperFect Reagent. (Data kindly provided by K. Kelly-Spratt, University of Texas Southwestern Medical Center, Dallas, TX, USA.)
Performance

SuperFect Reagent gives high transfection efficiency even when mixed with growth medium which can contain serum and antibiotics (see figure "Serum and DNA quantity vs. transfection efficiency"). SuperFect Reagent is suitable for transient and stable transfection of a broad range of cell lines (see figure "Transfection of neuronal PC-12 cells using SuperFect Reagent").

 

Principle

SuperFect Reagent consists of activated-dendrimer molecules with a defined spherical architecture. Branches radiate from a central core and terminate at charged amino groups which can then interact with negatively charged phosphate groups of nucleic acids (see figure "Activated dendrimer"). SuperFect Reagent assembles DNA into compact structures that bind to the cell surface and are taken into the cell by nonspecific endocytosis (see figure "SuperFect–DNA interaction"). The reagent buffers the pH of the endosome, leading to pH inhibition of endosomal nucleases, which ensures stability of SuperFect–DNA complexes. Due to highly controlled chemical synthesis the activated-dendrimer molecules in SuperFect Reagent have a precise size and a defined shape. This ensures consistent transfection-complex formation and reproducible transfection results.

Procedure

SuperFect Transfection Reagent is provided as a ready-to-use solution. The reagent is simply added to the DNA solution, mixed, and incubated for 5–10 minutes to allow SuperFect–DNA complexes to form. The complexes are mixed with growth medium (which can contain serum and antibiotics) and added directly to the cells. After a 2–3 hour incubation, a medium change is performed and the cells are incubated for expression of the transfected gene. Transfection using SuperFect Reagent requires minimal handling, making it highly suitable for high-throughput screening. SuperFect Reagent provides high transfection efficiencies, excellent reproducibility, and low cytotoxicity in high-throughput transfection, and is available in bulk quantities.

Applications

SuperFect Transfection Reagent is suitable for transient and stable transfection of a broad range of cell lines including 293, B16, BHK-21, COS-1, and CHO. SuperFect Reagent is suited for:

  • Studies on gene expression and function
  • Drug discovery
  • Development studies 

Specifications

Features
Specifications
Applications Plasmid transfection, protein overexpression
Cell type Eukaryotic cells
Controls Not included
Features Transfection in the presence of serum
Nucleic acid DNA
Number of possible transfections Up to 160 transfections in 12-well plates / 1.2 ml reagent
Technology Activated-dendrimer technology
Transfection type Stable transfection, transient transfection

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