DNeasy PowerBiofilm Kit

For the isolation of high-quality, pure DNA from biofilm samples

Features

  • High DNA yields from difficult biofilm samples
  • Inhibitor Removal Technology provides high-quality DNA for downstream applications
  • Optimized isolation of pure DNA from all biofilms, including dental plaques and microbial mats
DNeasy PowerBiofilm Sample (2)

Cat. No. / ID: 24000-S

Isolate high-quality, pure DNA from biofilm samples.
Preparations
2
50
The DNeasy PowerBiofilm Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

By pairing chemical and mechanical lysis techniques and Inhibitor Removal Technology (IRT), the DNeasy PowerBiofilm Kit overcomes inefficient cell lysis in the most challenging biofilms. IRT removes concentrated inhibitors such as humic acid, metals, salts and pesticides, typically present in biofilms resulting in increased yields of inhibitor-free DNA from all types of biofilms, including microbial mats. Want to try this solution for the first time? Request a quote for a trial kit. Purification of DNA using the DNeasy PowerBiofilm Kit can be automated on the QIAcube Connect.

DNeasy PowerBiofilm Kit was formerly sold by MO BIO as PowerBiofilm DNA Isolation Kit.

Performance



Supporting data and figures

Specifications

FeaturesSpecifications
FormatSilica Spin Filter Tubes
ProcessingBead Beating
Sample size0.05 - 0.20 g
Binding capacityUp to 20 µg per prep
Throughput1-24 samples
Time per run or per prep25 minutes
Storage temperatureStore at room temperature (15-30°C)
Sample typesAll processed biofilms from cultured single cell layers to microbial mats
Bead size0.1 mm glass, 0.5 mm glass, 2.4 mm ceramic mix

Resources

Quick-Start Protocols (1)
Kit Handbooks (1)

FAQ

Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699