QIAamp Investigator BioRobot Kit
适用于在BioRobot Universal System上自动纯化中到高通量的法医检材DNA
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QIAamp Investigator BioRobot Kit (12)
Cat. No. / ID: 965942
For 12 x 96 DNA preps: QIAamp 96 Plates, S-Blocks, Buffers, Proteinase K, Elution Microtubes CL, Caps, Disposable Troughs
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QIAamp Investigator BioRobot Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- 从法医检材和人类身份鉴定样本中纯化DNA
- 更有效地处理案件的痕量样本
- 高信噪比
- 快速纯化高品质、即用型DNA
Product Details
QIAamp Investigator BioRobot Kit使用经验证的QIAamp硅胶膜技术，可在BioRobot Universal System上自动纯化96个样本（如滤膜、案件或罪案样本、或血液），或192个拭子样本。自动化流程所需时间不到3小时，包括条形码输入，样本追踪和全过程记录，整个过程不需要手工操作。经纯化的DNA在下有分析中表现突出，如定量PCR和STR分析，具有很高的信噪比。
Performance
QIAamp Investigator BioRobot Kit适用于多种应用，包括：
- 从法医检材样本中纯化DNA
- 从不同的痕量样本中纯化DNA（如：罪案样本）
- 对照样本的常规分析
案件痕量样本的DNA产量高
自动纯化流程十分高效，DNA产量高（参见" Superior DNA yields"）。经纯化的DNA在下游分析中表现突出，如定量PCR和STR分析（参见" High-quality STR profiles"），具有很高的信噪比。该试剂盒每次可从24至96个法医检材样本中纯化高品质DNA。
See figures
Principle
BioRobot Universal System、Application Pack、Investigator和QIAamp Investigator Kit使用经过验证的QIAamp试剂，具有灵活、全自动、标准化流程的特点，可满足法医学实验室的工作需求。
Procedure
纯化流程可防止交叉污染。为提高便利性和灵活性，可选择自动样本裂解和DNA纯化；如果需要，也可以选择手动进行裂解，之后自动进行DNA纯化。BioRobot Universal System上全自动运行整个过程最多只需3小时，包括条形码输入、样本追踪和全过程记录，整个过程不需要手工操作。
Applications
QIAamp Investigator BioRobot Kit可从24到96个法医检材样本中纯化得到高品质DNA。使用该试剂盒，可在一次操作中处理以下多个类型的样本：
- 干燥血迹
- 表面拭子（如：血液、精液、唾液和表皮细胞）
- 衣物痕迹（如：沾有血液或唾液痕迹的衣物）
- 烟蒂或烟
- 口香糖和封套
Supporting data and figures
High-quality DNA from a wide range of sample types.
DNA was purified from the indicated sample types using the QIAamp Investigator BioRobot Kit (Investigator) on the BioRobot Universal System, or manually using the QIAamp DNA Micro Kit (Micro). Aliquots (5 μl, 10%) of each eluate were analyzed in triplicate by quantitative real-time PCR using the QuantiTect Probe PCR Kit with primers and probe specific for the β-actin gene. The mean threshold value (CT) and standard deviation are indicated for each sample.
Specifications
| Features | Specifications |
|---|---|
| Applications | STR Typing, PCR, blotting |
| Time per run or per prep | <3 hours |
| Yield | 1-2 µg DNA per swab; varying for casework samples |
| For automated processing | Biorobot Universal platform |
| Processing | Automated |
| Sample amount | 1 swab per well, up to 30 mg chewing gum, up to 40 mg cigarette butts, envelops, fabric or hair |
| Main sample type | Forensic casework and human identity samples |
| Elution volume | 150 µl |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Genomic DNA |
| Format | 96-well plate |
| Technology | Silica technology |
| Samples per run (throughput) | 24-96 samples per run |
Resources
安全数据表 (1)
试剂盒操作手册 (1)
Safety Data Sheets (1)
Certificates of Analysis (1)
FAQ
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?