Pyrosequencing Applications

Pyrosequencing is a unique detection technology based on the principle of sequencing-by-synthesis, which provides quantitative real-time data without the need for gels, probes, or labels. In addition to characterizing single nucleotide polymorphisms (SNPs), insertion-deletions (indels), and unknown sequence variants, it can sensitively quantify allele frequencies and DNA methylation levels at both CpG and non-CpG (CpN) sites. Integrating detection and quantification of genetic variation into one powerful system, Pyrosequencing with the PyroMark platform outperforms other sequence-based solutions in the analysis of targeted short DNA sequences. For a range of applications, this means reduced cost and time investment.

Sequencing technology has greatly expanded our understanding of how biological information is coded in the DNA sequence, but has also lead to new questions: What is the significance of subtle sequence changes, especially in non-coding regions? When do interactions of regulatory elements impact gene expression? How does nonsequence-based information — epigenetic information — contribute to developmental programming and cell differentiation? How can answers to these questions benefit applied sciences? These questions are the new horizons in genetic research. Researchers are uncovering new layers of biological information stored and inherited in genetic and epigenetic modifications of DNA. Characterization of this information requires next-generation research tools that enable application flexibility and acquisition of quantitative data. Pyrosequencing with QIAGEN PyroMark products meets these requirements.