Not sure which product to choose? Our helpful selection guides make decision making a lot easier!
Choose from the following:
| Speed |
Standard |
Fast |
Fast |
Standard |
| Kit name |
QIAGEN Plasmid Plus 96 Miniprep and BioRobot Kits |
QIAprep 96 Plus Miniprep and BioRobot Kits |
QIAprep 96 Turbo Miniprep and BioRobot Kits |
R.E.A.L. Prep 96 Plasmid Kit |
| Binding capacity |
<50 µg |
<50 µg |
<20 µg |
<30 µg |
| Culture volume (3–4 x 109 cells/ml) |
1.25 –5 ml |
1.25 –5 ml |
1–5 ml |
2.5 ml BAC/PAC/P1, 1.3 ml plasmid, 1.3–2.5 ml cosmid |
| Yield |
15–50 µg |
15–50 µg |
4–20 µg |
1 µg BAC/PAC/P1 |
| Elution volume |
80 µl (100–120 µl for a 5 ml culture) |
80 µl (100–120 µl for a 5 ml culture) |
100 µl |
22 µl BAC/PAC/P1, 50–250 µl plasmid, 50 µl cosmid |
| Procedure |
QIAvac 96, centrifuge, BioRobot Universal System |
QIAvac 96, centrifuge, BioRobot Universal System |
QIAvac 96, centrifuge, BioRobot Universal System |
QIAvac 96, centrifuge |
| Time |
50 min |
40 min |
45 min |
70 min |
| Speed |
Fast |
Fast |
Ultrafast |
| Kit name |
QIAGEN Plasmid Mini Kit |
QIAprep Spin Miniprep Kit |
Quicklyse Miniprep Kit |
| Binding capacity |
<20 µg |
<50 µg |
<15 µg |
| Culture volume (3–4 x 109 cells/ml) |
3 ml |
1–5 ml |
1–3 ml |
| Yield |
20 µg |
5–50 µg |
8–15 µg |
| Elution volume |
Variable, 50 µl recommended |
50 µl |
50 µl |
| Procedure |
Centrifuge, QIAcube |
QIAvac 24 Plus, centrifuge, QIAcube |
Centrifuge |
| Time |
80 min |
30 min |
9 min |
| Speed |
Standard |
Standard |
Fast
|
Ultrafast |
Ultrafast |
Standard |
Ultrafast |
| Kit name |
|
EndoFree Plasmid Kits |
QIAGEN Plasmid Kits |
QIAfilter Plasmid Kits |
QIAGEN Plasmid Plus Kits |
HiSpeed Plasmid Kits |
QIAGEN Large-Construct Kit |
CompactPrep Plasmid Kits |
| Culture volume (3–4 x 109 cells/ml) |
Midi |
_
|
25 ml, 100 ml
|
25 ml, 50–100 ml
|
20–25 ml, 25–35 ml (hy), 50 ml |
50 ml, 150 ml
|
500 ml |
25 ml, 50 ml |
| Maxi |
100 ml, 250 ml
|
100 ml, 500 ml
|
100 ml, 250 ml
|
80–100 ml, 100–130 ml (hy), 200 ml
|
150 ml, 250 ml
|
100 ml, 250 ml
|
| Mega |
500 ml/1.5 g, 2.5 l / 7.5g
|
500 ml/1.5 g, 2.5 l/ 7.5g
|
500 ml/1.5 g, 2.5 l/7.5g
|
500 ml
|
500 ml only rec for hc
|
– |
| Giga |
2.5 l/7.5 g, not rec. for lc
|
2.5 l/7.5 g, 5 l/15 g
|
2.5 l/7.5 g, not rec. for lc
|
2.5 l
|
2.5 l only rec for hc
|
– |
| Yield |
Midi |
–
|
75–100 µg, 20–100 µg
|
75–100 µg, 20–100 µg
|
100–200 µg, 150–250 µg (hy), 30–100 µg
|
100–200 µg, 30–100 µg
|
Up to 50 µg BAC/PAC/P1 DNA, up to 200 µg cosmid DNA |
100–200 µg, 30–100 µg
|
| Maxi |
300–500 µg, 50–250 µg
|
100–500 µg, 20–250 µg
|
300–500 µg, 50–250 µg
|
750 µg, 1 mg (hy), 50–250 µg
|
300–750 µg, 50–250 µg
|
350–750 µg, 50–250 µg
|
| Mega |
1.5–2.5 mg, 0.5–2.5 mg
|
1.5–2.5 mg, 0.5–2.5 mg
|
1.5–2.5 mg, 0.5–2.5 mg
|
Up to 2.5 mg
|
1.5–2.5 mg, endotoxin-free <0.1 EU/µg DNA
|
– |
| Giga |
7.5–10 mg
|
7.5–10 mg, 1–5 mg
|
7.5–10 mg
|
Up to 10 mg
|
7–10 mg, endotoxin-free <0.1 EU/µg DNA
|
– |
| Time |
Midi |
–
|
150 min
|
110 min
|
20 min
|
<45 min
|
280 min |
<20 min |
| Maxi |
150 min
|
160 min
|
120 min
|
20 min
|
<60 min
|
<20 min |
| Mega |
220 min
|
220 min
|
190 min
|
40 min
|
50 min
|
– |
| Giga |
310 min
|
320 min
|
280 min
|
50 min
|
50 min
|
– |
| Procedure |
Lysate clearing by precipitator or vacuum, gravity-flow |
Gravity-flow |
Lysate clearing by precipitator or vacuum, gravity-flow |
Vacuum and centriguge |
Gravity-flow and precipitator |
Centrifuge |
Centrifuge and vacuum |
lc = low copy,
hc =
high copy, hy = high yield; Sample size/volume: e.g., 500 ml / 1.5 g = pellet wet weight; rec = recommended