Single-Cell Mutation Detection – Overcoming Challenges in Single-Cell Analysis

Genetic alteration is the driving factor behind various biological processes such as developmental malformation, evolution and cancer. Recent findings of genomic heterogeneity among supposedly homogeneous cell populations such as tumor cells demands genomic characterization at the individual cell level to better understand the underlying biology. Single nucleotide variation (SNV) analysis by next-generation sequencing (NGS) requires relatively high coverage that often necessitates targeted enrichment or amplification to focus on sites of interest.

In this webinar, we will present a multiplex PCR-based targeted enrichment sequencing method for detecting mutations in single cells isolated from two colon cancer cell lines, Lovo and HT29. We will discuss the technologies along the workflow as well as the challenges and how to address them.