To ensure highest sensitivity, artus HI Virus-1 Kits have been optimized to detect low numbers of HIV-1 RNA. The analytical sensitivity of the artus HI Virus-1 QS-RGQ Kits is 76.4 IU/ml in consideration of the purification and assay setup using the QIAsymphony RGQ system (see figure " Highly sensitive detection of HIV-1 RNA"). (1 IU/ml corresponds to 0.45 copies/ml for detection of HIV-1 RNA on the Rotor-Gene Q. The conversion factor is an approximation based on an average factor across the assay's dynamic range.)
For highest specificity, validation of the artus HI Virus-1 Kits was carried out using various HIV-1 isolates, including all group M subtypes A–H, and related pathogens.
Kit | artus HI Virus-1 RG RT-PCR Kit | artus HI Virus-1 QS-RGQ Kit |
---|---|---|
Validated sample type | Human EDTA plasma | Human EDTA plasma |
Analytical sensitivity | 66.9 IU/ml | 76.4 IU/ml (corresponding to 34.4 copies/ml) |
Linear range | 120 to >1 x 108 IU/ml | 1 x 102 to 1 x 108 IU/ml (corresponding to 4.5 x 101 to 4.5 x 107 copies/ml) |
Specificity | HIV-1, including all group M subtypes A–H | HIV-1, including all group M subtypes A–H |
The artus HI Virus-1 RG RT-PCR Kit and the artus HI Virus-1 QS-RGQ Kit are based on the amplification and simultaneous detection of a specific region of the HIV-1 genome using real-time RT-PCR. The kits provide high levels of specificity, sensitivity (see figure " Highly sensitive detection of HIV-1 RNA"), and reproducibility over a broad linear range.
Each artus HI Virus-1 Kit provides 4 HIV-1 quantitation standards (see figure " Reliable quantitation of HIV-1 load"). Use of the standards enables accurate quantitation of viral load. In addition, the kits contains a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical HIV-1 PCR is not reduced.
Kit | artus HI Virus-1 RG RT-PCR Kit and artus HI Virus-1 QS-RGQ Kit |
---|---|
Validated sample type | Human EDTA plasma |
Amplicon | 93 nt region of the 5' LTR |
artus HI Virus-1 RT-PCR Kits provide all necessary reagents optimized for reliable HIV-1 RNA detection and quantitation. Simply add template RNA to the ready-to-use PCR master mix, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.
The QIAsymphony RGQ workflow solution for HIV-1 detection comprises the QIAsymphony SP for sample preparation, the QIAsymphony AS for assay setup, and the artus HI Virus-1 QS-RGQ Kit on the Rotor-Gene Q. The system enables reliable pathogen detection with a complete IVD workflow (see figure " Integrated QIAsymphony RGQ system for HIV-1 detection").
The artus HI Virus-1 RG RT-PCR Kit is validated for use with viral RNA purified from human plasma using the IVD-validated QIAamp DSP Virus Kit.
The artus HI Virus-1 RG RT-PCR Kit enables rapid and sensitive detection and quantitation of HIV-1 RNA purified from human plasma using the QIAamp DSP Virus Kit.
The artus HI Virus-1 QS-RGQ Kit is designed to be used with the QIAsymphony RGQ system, providing a complete IVD workflow from sample to HIV-1 RNA detection and quantitation.
Features | Specifications |
---|---|
Recommended sample prep | QIAamp DSP Virus Kit |
Quantitative/qualitative | Quantitative |
Sample type | EDTA plasma |
RUO/CE/ASR | CE |
What detected | Human Immunodeficiency Virus type 1 RNA |
Thermal cycler | Rotor-Gene 6000 |