Absolute quantification of miRNAs and CNVs with high accuracy and precision using digital PCR
MicroRNAs (miRNAs) are short non-coding RNAs, which are shown to post-transcriptionally regulate gene expression. Since miRNAs are present in both tissues and body fluids, they have a great potential as non-invasive diagnostic and prognostic biomarkers. Deregulation of miRNA expression has been found to contribute significantly to the pathogenesis, progression and prognosis of many types of cancer. miRNA expression in malignant cells is often related to alterations in miRNA copy numbers and gene locations. In addition to miRNAs, copy number variations (CNVs) have been identified in various cancer types. Over the past few decades, cancer research has made tangible advances. However, robust quantification of miRNA and CNV can be difficult, especially in samples with a high inhibitory burden or low nucleic acid content. Digital PCR using the QIAcuity system allows you to overcome these limitations and detect and quantify miRNAs and CNVs with high sensitivity and precision.
This webinar will cover:
- Accurate and precise quantification of miRNAs using dedicated assays on the QIAcuity
- Multiplex detection of up to five CNVs using dedicated assays on the QIAcuity