The power of LNA enhancement
Locked nucleic acids (LNA) are a class of high-affinity RNA analogs in which the ribose ring is "locked" into the ideal conformation for Watson-Crick binding. These oligonucleotides have substantially increased affinity for their complementary strand, when compared with traditional DNA or RNA oligonucleotides. This results in unprecedented sensitivity and specificity and makes LNA-enhanced oligos excellent tools for detecting and differentiating small or highly similar DNA or RNA targets in many research applications. Discover more about LNA in our Learning Hub.
Unique systems developed specifically for miRNA profiling
Both the miRCURY LNA miRNA PCR System, which uses SYBR Green for detection, and the new probe-based miRCURY LNA miRNA Probe PCR System offer the best available combination of performance and ease-of-use on the market through a unique combination of features, but differ in detection strategy.
- Both systems use one cDNA reaction for all miRNAs – A single universal first-strand cDNA synthesis reaction is used as the template, regardless of the number of miRNAs being profiled. This saves precious sample, reduces technical variation, minimizes pipetting and saves time in the laboratory.
- The miRCURY LNA miRNA PCR System uses two LNA-enhanced, miRNA-specific qPCR primers and SYBR Green for the signal detection (see Schematic outline of the miRCURY LNA miRNA PCR System). The miRCURY LNA miRNA Probe PCR System uses an LNA-enhanced, miRNA-specific forward primer and an LNA-enhanced, miRNA-specific hydrolysis probe (see miRCURY LNA miRNA Probe PCR System at a glance). LNA-enhancement ensures that the primers of both systems and the probe of the latter bind with high affinity. Primers and probe are also shorter than standard PCR primers, so they can be designed to be fully miRNA-specific without overlapping. The result is unrivalled sensitivity and specificity and extremely low background.
Unmatched sensitivity
Fully validated and optimized
Truly specific – no false positives
Fast, easy and reproducible
Flexible qPCR system
What you need for each system
Detection system | SYBR Green | LNA-enhanced hydrolysis probe |
|
---|---|---|---|
1 | Reverse Transcription | miRCURY LNA RT Kit | miRCURY LNA RT Kit |
2 | Optional spike-in | RNA Spike-In Kit, For RT | RNA Spike-In Kit, For RT |
3 | Mastermix for qPCR | miRCURY LNA SYBR Green PCR Kits | miRCURY LNA Probe PCR Kits |
4 | Assay or panel | • miRCURY LNA miRNA PCR Assays • miRCURY LNA miRNA Custom PCR Assays • miRCURY LNA miRNA Focus PCR Panels • miRCURY LNA miRNA Custom PCR Panels • miRCURY LNA miRNA miRNome PCR Panels |
• miRCURY LNA miRNA Probe PCR Assays Coming soon: |