For RNA cleanup and concentration with small elution volumes
  • Efficient cleanup of enzymatic reactions
  • Concentration of small amounts of RNA to only 10 µl
  • Cleanup of RNA purified by different methods
  • High-quality RNA in less than 15 minutes

The RNeasy MinElute Cleanup Kit enables cleanup and concentration of RNA from enzymatic reactions or other samples using specialized RNeasy MinElute spin columns based on silica-membrane technology. The kit can also be used to desalt RNA samples. Up to 45 μg RNA can be purified in a volume as low as 10 μl. Purification can be fully automated on the QIAcube.

Quantity Product Cat. no. Price
 
 
show details
  varies
Quantity Product Cat. no. Price Sum
 
RNeasy MinElute Cleanup Kit (50)
50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers
74204
$312.00
$0.00
The RNeasy MinElute Cleanup Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
  • Main Image Navi
High-quality RNA.|Concentration of RNA.|Reliable cleanup of RNA.|Efficient removal of real-time RT-PCR inhibitors.|
Total RNA was isolated from HeLa cells using an acid-phenol method plus cleanup and concentration using the RNeasy MinElute Cleanup Kit. The high quality of the RNA is shown by scanning with the Agilent 2100 Bioanalyzer.|The indicated amounts of total RNA from HeLa cells were concentrated using the RNeasy MinElute Cleanup Kit, and 5 µl of the 12 µl eluate was used for each reaction. Real-time RT-PCR was carried out on an ABI Sequence Detection System using the QuantiTect Probe RT-PCR Kit, with primers and probes specific for β-actin.|The indicated amounts of total RNA from HeLa cells were concentrated using the RNeasy MinElute Cleanup Kit. Real-time RT-PCR was carried out on the LightCycler System using the QuantiTect Probe RT-PCR Kit, with primers and probes specific for the p16 gene.|Without cleanup, inhibitors can lead to underestimation of RNA amounts in real-time quantification. Total RNA was isolated from HeLa cells using an acid-phenol method, with or without subsequent cleanup using the RNeasy MinElute Cleanup Kit. Real-time RT-PCR was carried out as described in figure "Concentration of RNA", with the indicated volumes of the RNA eluate. Apparent RNA amounts were calculated from the resulting CT values.|
Performance
The RNeasy MinElute Cleanup Kit provides high-quality total RNA, free from impurities or enzymatic inhibitors, with A260/A280 ratios of 1.9–2.1 (see figure "High-quality RNA"). RNA amounts corresponding to less than one cell (as little as 1 pg) can be concentrated (see figure "Concentration of RNA"). A large amount of RNA (up to 45 µg) can be purified and is suitable for use in sensitive assays (see figure "Reliable cleanup of RNA").  Reaction volumes can be kept small in downstream applications, giving increased reaction efficiency. The high purity RNA allows more of the sample to be used in reactions without inhibition (see figure "Efficient removal of real-time RT-PCR inhibitors").
Principle

The RNeasy MinElute Cleanup Kit is designed to purify and concentrate RNA from enzymatic reactions (e.g., labeling, in vitro transcription), RNA isolated by alcohol-precipitation and organic-extraction methods, as well as to desalt RNA samples, and concentrate RNA prepared by silica-membrane procedures (e.g., PAXgene Blood RNA preps). RNeasy technology simplifies total RNA isolation by combining guanidine-isothiocyanate lysis with silica-membrane purification.

Procedure

Guanidine-isothiocyanate–containing lysis buffer and ethanol are added to the sample to create conditions that promote selective binding of RNA to the RNeasy MinElute membrane. The sample is then applied to the RNeasy MinElute spin column. RNA binds to the silica-membrane, contaminants are efficiently washed away, and high-quality RNA is eluted in water.

Enzymatic reactions or crude RNA preps are simultaneously cleaned up and concentrated in less than 15 minutes. In comparison, time-consuming concentration and cleanup by alcohol precipitation can result in loss of RNA, especially from small samples. The RNeasy MinElute procedure is faster than vacuum centrifugation, which only concentrates the sample without removing salts and other impurities. The unique design of RNeasy MinElute spin columns enables concentration of purified RNA to as little as 10 µl for downstream applications such as microarray analysis and real-time RT-PCR. Purification can be fully automated on the QIAcube.

Applications

RNA purified with RNeasy MinElute technology is high-quality and ideal for use in all applications including:

Northern, dot, and slot blotting
End-point RT-PCR
Quantitative, real-time RT-PCR
Array analysis
Poly A+ RNA selection
Feature
Specifications
Applications PCR, qPCR, real-time PCR, microarray
Elution volume 10–14 µl
Format MinElute Spin column
Main sample type (Crude) RNA preps
Processing Manual
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein RNA
Sample amount 200 µl
Technology Silica technology
Time per run or per prep <15 minutes
Yield 45 µg

You are not authorized to download the resource

FAQs
40
FAQ ID -286
View
FAQ ID -139
View
FAQ ID -1212
View
FAQ ID -290
View
FAQ ID -1616
View
FAQ ID -514
View
FAQ ID -97
View
FAQ ID -490
View
FAQ ID -2800
View
FAQ ID - 3388
View

View
FAQ ID -1087
View
FAQ ID -432
View
FAQ ID -1043
View
FAQ ID -429
View
FAQ ID -745
View
FAQ ID -1024
View
FAQ ID -1023
View
FAQ ID -2793
View
FAQ ID -528
View
FAQ ID -12
View
FAQ ID -2661
View
FAQ ID -32
View
FAQ ID -28
View
FAQ ID -159
View
FAQ ID -2248
View
FAQ ID -813
View
FAQ ID -113
View
FAQ ID -753
View
FAQ ID -430
View
FAQ ID -728
View
FAQ ID -2797
View
FAQ ID -101
View
FAQ ID -1026
View
FAQ ID -619
View
-20
View
FAQ ID -103
View
FAQ ID -1258
View
FAQ ID -2796
View
FAQ ID -1619
View
Kit Handbooks
2
Supplementary Protocols
2