MagAttract Viral RNA Kit
For hands-free isolation of RNA from respiratory-swab samples using automated processing and liquid handling systems
Products
The MagAttract Viral RNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Features
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Automated high-throughput isolation of viral RNA
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Up to 960 respiratory human swab samples in less than one day
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Optimized for use with the Thermo Scientific KingFisher Flex platform
Product Details
The MagAttract Viral RNA Kit combines the speed and efficiency of silica-based nucleic acid purification with the convenient handling of magnetic particles. A well-established technology is used for viral RNA preparation in a 96-well format. The purification procedure is designed to ensure reproducible handling and comprises four steps: lyse, bind, wash and elute.
Principle
The MagAttract Viral RNA Kit provides fully automated purification of viral RNA from respiratory swab samples. MagAttract technology combines the speed and efficiency of silica-based RNA purification with the convenient handling of magnetic particles. Samples are processed in the KingFisher Flex Workdeck. No phenol–chloroform or manual column extraction is required.
Procedure
In the automated workflow, viral RNA binds specifically to the magnetic beads while contaminants such as divalent cations and proteins, are completely removed in three efficient wash steps. High-quality RNA is eluted in a special RNase-free buffer, ready for direct use or safe storage. The purified RNA is free of proteins, nucleases, and other contaminants or inhibitors and can be used immediately in demanding downstream applications, including PCR, qPCR, dPCR and next-generation sequencing. Protocols are available for use on the Thermo Scientific KingFisher Flex. This kit can also be used with many other automated handling systems.
Carrier RNA
Carrier RNA serves two purposes: First, it enhances binding of viral nucleic acids to the magnetic beads, especially if there are very few target molecules in the sample. Secondly, the addition of large amounts of carrier RNA reduces the chance of viral RNA degradation in the rare event that RNase molecules escape denaturation. Not adding carrier RNA to Buffer ACL may lead to reduced viral RNA recovery.
Applications
The high-quality RNA obtained using the MagAttract Viral RNA Kit and the Thermo Scientific KingFisher Flex platform is suited for use in many downstream applications, such as:
- Viral epidemiology and genotyping
- Infectious disease research
Supporting data and figures
Recovery of SARS-CoV-2 RNA from respiratory swab supernatants.
RNA purified from respiratory swab supernatant using the indicated kits was subjected to RT-PCR (mean value and deviation of threshold cycle shown). (A) negative samples spiked with AccuPlex SARS-CoV-2; (B) 1:10 diluted SARS-CoV-2 positive samples.
Specifications
| Features | Specifications |
|---|---|
| Applications | PCR, real-time PCR, digital PCR, genotyping, sequencing |
| Sample amount | 300 µl |
| For automated processing | KingFisher Flex Workdeck |
| Elution volume | 80 µl |
| Main sample type | Respiratory swabs |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Viral RNA |
| Processing | Automated |
| Format | 96 well |
| Technology | Magnetic particles |
Resources
Safety Data Sheets (1)
Certificates of Analysis (1)
FAQ
Where can I download the protocol for the KingFisher Flex?
Who can help if it is not clear if issues are caused by the third party instrument or by the chemistry?
Who can help in case of issues with the third party instrument?
Is the virus inactivated during the procedure?
I would like to use the kit on another third party instrument. Who should I contact?
Can I use sample volumes other than 300 µl?
Are protocols also available for other instruments (e.g., Hamilton Star and Tecan Fluent)?