GeneRead DNAseq Gene Panels are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
GeneRead DNAseq Gene Panel System targeted enrichment NGS workflow.|Multiplex PCR-enabled target enrichment of genes of interest|
First, extract the DNA (the QIAamp DNA Mini Kit or QIAamp DNA FFPE Tissue Kit is recommended) and then use the GeneRead DNAseq Gene Panels for targeted exon enrichment. Then construct your NGS library, quantify and quality-control using the GeneRead Library Quantification System, and perform NGS and data analysis using the QIAGEN NGS Data Analysis Web Portal.|The principle of the GeneRead DNAseq System is to employ overlapping primer sets across the exonic portions of a gene or genes to maximize target coverage and minimize nonspecific amplification.|
The performance of GeneRead DNAseq Gene Panels is assessed by three criteria: design coverage, specificity, and coverage uniformity. Greater than 90% of exonic regions are covered by the panel primer design, enhancing potential variant discovery. The high specificity of the system maximizes efficient use of sequencing capacity, as more than 85% of sequencing reads align to target regions. Finally, the high coverage uniformity of the system, with more than 85% of targeted bases covered at >10% median sequencing depth, ensures high-quality variant calls.
The GeneRead DNAseq Gene Panel System employs overlapping primer sets across the exonic portions of a gene or group of genes to maximize target coverage (see Multiplex PCR-enabled target enrichment of genes of interest
). Overlapping primer sets are divided into 4 tubes, thereby increasing the specificity of coverage without primer dimers and nonspecific amplifications. Following amplification and purification, enriched regions from each sample are pooled together, yielding one library preparation for each sample.
GeneRead DNAseq Gene Panels are part of a total workflow for targeted next-generation sequencing (see figure, GeneRead DNAseq Gene Panel System targeted enrichment NGS workflow
). Simply extract DNA from your samples (the QIAamp DNA Mini Kit or the QIAamp DNA FFPE Tissue Kit are recommended), and then use the GeneRead DNAseq Gene Panels in combination with GeneRead Panel Mastermix to perform targeted exon enrichment using PCR. Once targets have been enriched, construct the NGS library and use the GeneRead DNAseq Library Quant Array to quantify and perform quality control. Then perform NGS and analyze your data using the QIAGEN NGS Data Analysis Web Portal.
GeneRead DNAseq Gene Panels are highly suited for enriching target genes specific for various cancers, inheritance diseases, neurological diseases, cardiomyopathy, development disorders, metabolic disorders, and many other areas of biomedical research to determine genetic variants through NGS.