For multiplex amplification of 30 deletion/insertion polymorphisms plus Amelogenin

  • Unique kit featuring alternative markers for human identification
  • Highly suited for degraded DNA thanks to ~160 bp amplicon length
  • Suitable for disaster victim identification and anthropological studies
  • Easy interpretation with dedicated freeware DIPSorter

The Investigator DIPplex Kit enables the amplification of 30 deletion/insertion polymorphisms (also known as INDELs) plus Amelogenin. This provides an equally sensitive alternative to STR- and SNP-based human identification. The kit is designed to analyze highly degraded DNA, thanks to the maximum amplicon size of just ~160 bp. The genetic analysis is calibrated with BT5 Matrix, an optimized dye technology.

Quantity Product Cat. no. Price
 
 
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Please note: 384013: Investigator DIPplex Kit (25) is no longer available and has been superseded by the 384015: Investigator DIPplex Kit (100). For more information, please contact QIAGEN Technical Services.
Quantity Product Cat. no. Price Sum
 
Investigator DIPplex Kit (25)
Primer Mix, Reaction Mix, Multi Taq2 Polymerase, Control DNA, DNA Size Standard 550 (BTO), Allelic Ladder DIPplex, and Nuclease-Free Water
384013
Inquire
 
Investigator DIPplex Kit (100)
Primer Mix, Reaction Mix, Multi Taq2 Polymerase, Control DNA, DNA Size Standard 550 (BTO), Allelic Ladder DIPplex, and Nuclease-Free Water
384015
$1,526.00
$0.00

The Investigator DIPplex Kit is intended for molecular biology applications in human identity testing. This product is not intended for the diagnosis, prevention, or treatment of a disease. USA and Canada: Not for use in, or in preparation for, legal proceedings, including parentage determination.


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Performance

The Investigator DIPplex Kit was validated and evaluated using the GeneAmp 9700 thermal cycler (in standard mode), and the ABI PRISM 310, ABI PRISM 3100, and Applied Biosystems 3130 Genetic Analyzers. 

The recommended amount of DNA under standard conditions is 0.35 ng. Internal validations demonstrated robust and balanced results with 0.2–0.5 ng, and reliable results with <0.1 ng DNA.

The Investigator DIPplex Kit has significant advantages over standard STR- and SNP-based DNA profile generation, including the absence of stutter peaks and the very short amplicons (under 160 bp).

Principle

Human identification is commonly based on the analysis of short tandem repeats (STRs) or single nucleotide polymorphisms (SNPs), depending on the demands of an examination or on the sample quality. The Investigator DIPplex Kit uses an alternative means of human identification by allowing the analysis of biallelic deletion/insertion polymorphisms (DIPs, also called INDELs).

Procedure

The 30 biallelic DIP loci plus Amelogenin are simultaneously amplified in a single PCR and labeled with four dyes. See the table "Technical specifications" for more information.

Technical specifications
Volume per amplification reaction 25 μl
Matrix BT5
Fluorescence labels 6-FAM, BTG, BTY, BTR, BTO
Genetic analyzers ABI PRISM 310, ABI PRISM 3100/3100-Avant, Applied Biosystems 3130/3130xl, or Applied Biosystems 3500/3500xL Genetic Analyzers
Software for analysis Applied Biosystems GeneMapper ID, GeneMapper ID-X, or Genotyper Software
Applications

The Investigator DIPplex Kit is for generation of DNA profiles from forensic stains, hair, and bone samples, and it is highly suitable for:

Disaster victim identification
Anthropological studies
Mixed stain analysis
Other human identity testing applications

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