Oxidative Stress
Find more about Oxidative Stress
An imbalance between the production of reactive oxygen species (ROS) and the detoxification of their reactive intermediates causes oxidative stress. Cells must respond to this imbalance before the highly reactive molecules damage cellular structures, particularly DNA. Severe and prolonged oxidative stress can trigger apoptosis and necrosis. Numerous pathological conditions have an oxidative stress component, including cardiovascular diseases, neurodegenerative diseases such as Alzheimer’s disease, and chronic inflammation. Signaling pathways downstream of ROS detection, such as PKC, PI3K, and MAPK, phosphorylate the transcription factor nuclear factor-erythroid 2-related factor 2 (Nrf2). This transcription factor binds the antioxidant response element (ARE) within the promoters of genes encoding antioxidant enzymes and detoxifying enzymes. Key Nrf2 target genes include antioxidants such as glutathione peroxidases (GPx) and genes involved in superoxide metabolism. These genes reduce oxidative stress by breaking down ROS. Analysis of oxidative stress mechanisms may yield new insights into the dysregulation of this biological process during disease processes. ...
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An imbalance between the production of reactive oxygen species (ROS) and the detoxification of their reactive intermediates causes oxidative stress. Cells must respond to this imbalance before the highly reactive molecules damage cellular structures, particularly DNA. Severe and prolonged oxidative stress can trigger apoptosis and necrosis. Numerous pathological conditions have an oxidative stress component, including cardiovascular diseases, neurodegenerative diseases such as Alzheimer’s disease, and chronic inflammation. Signaling pathways downstream of ROS detection, such as PKC, PI3K, and MAPK, phosphorylate the transcription factor nuclear factor-erythroid 2-related factor 2 (Nrf2). This transcription factor binds the antioxidant response element (ARE) within the promoters of genes encoding antioxidant enzymes and detoxifying enzymes. Key Nrf2 target genes include antioxidants such as glutathione peroxidases (GPx) and genes involved in superoxide metabolism. These genes reduce oxidative stress by breaking down ROS. Analysis of oxidative stress mechanisms may yield new insights into the dysregulation of this biological process during disease processes.

QIAGEN provides a broad range of assay technologies for oxidative stress research that enable analysis of gene expression and regulation, epigenetic modification, genotyping, and signal transduction pathway activation. Solutions optimized for oxidative stress studies include PCR array, miRNA, siRNA, mutation analysis, pathway reporter, chromatin IP, DNA methylation, and protein expression products.
  • Description
RT² Profiler PCR Array
The Human Oxidative Stress RT² Profiler PCR Array profiles the expression of 84 genes related to oxidative stress. Peroxidases are represented on this array including glutathione peroxidases (GPx)...

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Species
Human
Cat. no.
PAHS-065Z
RT² Profiler PCR Array
The Mouse Oxidative Stress RT² Profiler PCR Array profiles the expression of 84 genes related to oxidative stress. Peroxidases are represented on this array including glutathione peroxidases (GPx)...

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Species
Mouse
Cat. no.
PAMM-065Z
RT² Profiler PCR Array
The Rat Oxidative Stress RT² Profiler PCR Array profiles the expression of 84 genes related to oxidative stress. Peroxidases are represented on this array including glutathione peroxidases (GPx)...

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Species
Rat
Cat. no.
PARN-065Z
Cignal Reporter Assay Kit
The Cignal ARE Reporter Assay Kit is designed to measure the transcriptional activity of Nrf2 (NF-E2-related factor 2) and Nrf1 transcription factors. The Nrf2 and Nrf1 bind to antioxidant...

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Species
Luciferase
Cat. no.
CCS-5020L
Cignal Reporter Assay Kit
The Cignal Antioxidant Response (ARE) Reporter Assay Kit is designed to monitor the activity of Nrf2 and Nrf1 transcription factors in cultured cells. Nrf2 and Nrf1 bind to the antioxidant...

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Species
GFP
Cat. no.
CCS-0020G
Cignal Lenti Reporter Assay
The Cignal Lenti Reporter Assays are self-inactivating replication incompetent viral particles. The lentiviral particles are ready to add directly to your cells. The Cignal Lenti ARE Reporter is...

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Species
Luciferase
Cat. no.
CLS-2020L
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