For purification of total RNA from plants and fungi
  • High-quality total RNA in 30 minutes
  • No phenol/chloroform extraction
  • No CsCl gradients, no LiCl or ethanol precipitation
  • Excellent recovery of RNA
  • Ready-to-use RNA for any downstream application

The RNeasy Plant Mini Kit includes QIAshredder spin columns for homogenizing and filtering viscous plant or fungal lysates, and RNeasy spin columns for purifying up to 100 μg of high-quality RNA using silica-membrane technology. Purification can be fully automated on the QIAcube. The kit can also be used in combination with a TissueRuptor or TissueLyser system, which provide efficient disruption and homogenization of plant samples.

Quantity Product Cat. no. Price
 
 
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Quantity Product Cat. no. Price Sum
 
RNeasy Plant Mini Kit (20)
20 RNeasy Mini Spin Columns, 20 QIAshredder Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers
74903
$141.00
$0.00
 
RNeasy Plant Mini Kit (50)
50 RNeasy Mini Spin Columns, 50 QIAshredder Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers
74904
$333.00
$0.00
The RNeasy Plant Mini Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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RNeasy Plant Mini procedure.|Detection of viruses.|Tissue specificity of histone H4 expression.|
|Total RNA was isolated from budwood obtained from infected apple (1), cherry (2), pear (3), and grapevine (4–8), leaf tissue from black currant (9, 10), and dormant tuber tissue from infected potato (11, 12) using a modification of the RNeasy protocol. Viral RNA was amplified using a one-tube RT-PCR. Amplification products were analyzed on a 1.5% agarose gel. M: DNA Markers VI, Boehringer Mannheim. (Data kindly provided by D.J. MacKenzie, Centre for Plant Health, Agriculture and Agri-Food Canada and M.A. McLean, Euro Nursery & Vineyard (West) Inc., Sidney, Canada.)|Formaldehyde agarose gel and northern blot of total RNA (7 µg) from indicated tissues of tomato. Blot was hybridized with a 32P-labeled DNA probe for the tomato histone H4 gene. M: 0.24-9.5 kb RNA ladder. (Plant tissues and histone H4 cDNA clone kindly provided by K. Theres, MPI for Breeding Research, Cologne, Germany.)|
Performance

The RNeasy Plant Mini Kit is ideal for isolation of total RNA from a wide variety of plant and fungal samples with sample sizes of 10–100 mg tissue, or 100–1 x 107 cells (see table "Selected samples processed with the RNeasy Plant Mini Kit"). The binding capacity of the spin-columns is up to 100 µg RNA. The typical yield from 100 mg plant tissue is between 25 µg and 65 µg of RNA, although amounts of RNA can vary due to the developmental stage and growth conditions of the samples (see table "Yield from 100 mg tissue").

Selected samples processed with the RNeasy Plant Mini Kit
PlantsFilamentous fungi
Anemone sp.
Arabidopsis thaliana1
Begonia sp.
Beta vulgaris (sugar beet)2
Chlamydomonas reinhardii (unicellular alga)
Chrysanthemum
Clarkia spp.3
Daucus carota (carrot)4
Diascia sp.
Dendranthema sp.
Euglena gracilis (unicellular alga)
Funaria hygrometrica (moss)
Hordeum vulgare (barley)
Lycopersicon esculentum (tomato)5
Malus sp. (apple)6
Nicotiana tabacum (tobacco)
Oryza sativa (rice)
Pelargonium sp. (geranium)
Petroselinum crispum (parsley)7
Pisum sativum (pea)
Prunus sp. (cherry)6
Ranunculus sp.
Ribes nigrum (black currant)
Riccia fluitans (liverwort)
Sinapis arvensis (mustard)
Solanum tuberosum (potato)8
Spinacia oleracea (spinach)
Surfinia sp.
Triticum aestivum (wheat)
Vitis sp. (grapevine)6
Zea mays (maize)
Acremonium crysogenum9
Fusarium avenaceum9
Young leaves or needles, except as indicated: 1 Flowers, buds, siliques, roots; 2 roots; 3 petals, stamen, stigma, sepals, style; 4 cultured cells; 5 mature flower, expanded leaves, shoot tip, stem, green fruit; 6 budwood; 7 seedlings; 8 dormant tuber; 9 mycelium

 

Yield from 100 mg tissue
Sample typeYield
Arabidopsis leaves 35 µg
Maize leaves 25 µg
Tomato leaves 65 µg
Tobacco leaves 60 µg

The purified plant RNA, eluted in 30–100 µl volume, includes viral RNA (see figure "Detection of viruses"). All contaminants, such as polysaccharides, are removed, and the eluted RNA is ready for all downstream applications (see figure "Tissue specificity of histone H4 expression").

Principle

The RNeasy Plant Mini Kit provides QIAshredder columns for homogenization and filtration of viscous plant or fungal lysates by microcentrifugation in combination with RNeasy Mini spin columns for RNA purification. RNeasy technology simplifies total RNA isolation by combining the stringency of guanidine-isothiocyanate lysis with the speed and purity of silica-membrane purification. RNeasy Kits provide the highest-quality RNA with minimum copurification of DNA. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using convenient on-column DNase treatment during the RNeasy procedure.

Procedure
Samples are first lysed and then homogenized in the QIAshredder columns. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the RNeasy silica membrane. RNA binds, and all contaminants are efficiently washed away. Pure, concentrated RNA is eluted in water (see flowchart "RNeasy Plant Mini procedure").
Applications

RNA purified with RNeasy technology is high-quality and is ideal for use in all applications. Downstream applications include:

Northern, dot, and slot blotting
End-point RT-PCR
Quantitative, real-time RT-PCR
Array analysis
Poly A+ RNA selection
Feature
Specifications
Applications PCR, qPCR, real-time RT-PCR, microarray
Elution volume 30–100 µl
Format Spin column
Main sample type Plant samples
Processing Manual
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein RNA
Sample amount 10–100 mg
Technology Silica technology
Time per run or per prep 30 minutes
Yield 25–60 µg

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Kit Handbooks
1
RNeasy Mini Kit - For purification of total RNA from animal cells, animal tissues, bacteria, and yeast, and for RNA cleanup; RNeasy Protect Mini Kit - For immediate stabilization of RNA in harvested animal tissues and subsequent total RNA purification; RNeasy Plant Mini Kit - For purification of total RNA from plants and filamentous fungi
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