For immediate stabilization and convenient transport of sorted or cultured cells
  • Immediate RNA stabilization with no need to remove medium
  • Cells stable at 30ºC (1 day), 15–25ºC (7 days), or 2–8ºC (4 weeks)
  • Archiving of cells at –20ºC or –80ºC

RNAprotect Cell Reagent provides immediate stabilization of RNA in sorted or cultured cells. Cells are stabilized at room temperature and can be stored or transported at ambient temperature prior to RNA purification.

Quantity Product Cat. no. Price
 
 
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Quantity Product Cat. no. Price Sum
 
RNAprotect Cell Reagent (250 ml)
250 ml RNAprotect Cell Reagent
76526
$302.00
$0.00
The RNAprotect Cell Reagent is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Effective inhibition of PMA induction of c-fos expression.|More reliable quantification of CD83 transcript.|
Incubation of PMA-stimulated Jurkat cells in RNAprotect Cell Reagent prevents PMA induction of c-fos expression as well as degradation of c-fos transcript, demonstrating stabilization of RNA by RNAprotect Cell reagent (each bar represents the mean of duplicate cell samples).|Storage of cytokine-stimulated immature dendritic cells overnight at –20ºC in RNAprotect Cell Reagent instead of PBS allows quantification of higher levels of CD83 mRNA, indicating stabilization of RNA by RNAprotect Cell Reagent. (Data kindly provided by Dr Alexander Prechtel and Prof. Alexander Steinkasserer, University Hospital Erlangen, Erlangen, Germany.)|
Performance
RNAprotect Cell Reagent prevents induction of gene expression in treated cells and preserves and stabilizes transcript levels during storage of cell samples (see figures " Effective inhibition of PMA induction of c-fos expression" and " More reliable quantification of CD83 transcript").
Principle

RNAprotect Cell Reagent provides immediate stabilization of RNA in sorted or cultured cells. Cells are stabilized at room temperature and can be stored or transported at ambient temperature prior to RNA purification.

Procedure
RNAprotect Cell Reagent immediately stabilizes the gene expression profile in cells. With other technologies, it is necessary to wash or process the cells (e.g., trypsin treatment) prior to adding an RNA stabilization reagent. Changes in expression that may occur during the washing or processing steps are eliminated by adding RNAprotect Cell Reagent directly to cells in culture medium or buffer. Instantaneous RNA stabilization with RNAprotect Cell Reagent can also be extended to pelleted cells not in medium or storage solution. The amount of starting material must not exceed 1 x 107 cells.
Applications

Cells in RNAprotect Cell Reagent can be conveniently stored and transported at ambient temperature before further processing and RNA purification. The RNA can be purified using the RNeasy Plus Mini Kit, or purified simultaneously with DNA from the same cell sample using an AllPrep Kit.

Kit Handbooks
2
RNAprotect Cell Reagent ─ 培養細胞中のRNAを迅速に安定化; RNeasy Protect Cell Mini Kit ─ 培養細胞中のトータルRNAの迅速な安定化およびトータルRNA精製  
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RNAprotect Cell Reagent
For immediate stabilization of nucleic acids in cells

RNeasy Protect Cell Mini Kit
For immediate stabilization of nucleic acids in cells
and subsequent total RNA purification
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