- Up to 300 μg His-tagged protein per column in as little as 15 minutes
- Purification under native and denaturing conditions
- Up to 95% homogeneity in one step
- Ready-to-use spin columns for rapid automated or manual processing
Ni-NTA silica combines Ni-NTA with a macroporous silica support material optimized to suppress nonspecific hydrophobic interactions. The Ni-NTA Spin Columns provide Ni-NTA silica in a convenient microspin format for easy preparation of multiple samples in parallel. They provide a simple method for functional screening of engineered proteins, selection of clones expressing full-length translation products, and comparison of expression levels. Each spin column can purify up to 300 µg of His-tagged protein. Like all Ni-NTA matrices, Ni-NTA spin columns can be used for one-step protein purification under native or denaturing conditions.