EasyXpress Protein Synthesis Kits

For in vitro synthesis of recombinant proteins in high yields
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Please note: product 32502: EasyXpress Protein Synthesis Kit (20) has been discontinued as of December 31, 2013. From January 01, 2014 an equivalent product will be sold by RiNA GmbH, Berlin. For more information, please contact www.rina-gmbh.eu or +49 30 844 166 0

product 32506: EasyXpress Protein Synthesis Maxi Kit has been discontinued as of December 31, 2013. From January 01, 2014 an equivalent product will be sold by RiNA GmbH, Berlin. For more information, please contact www.rina-gmbh.eu or +49 30 844 166 0

product 32501: EasyXpress Protein Synthesis Kit (5) has been discontinued as of December 31, 2013. From January 01, 2014 an equivalent product will be sold by RiNA GmbH, Berlin. For more information, please contact www.rina-gmbh.eu or +49 30 844 166 0
Quantity Product Cat. no. Price Sum
 
EasyXpress Protein Synthesis Kit (5)
For 5 x 50 µl reactions: E. coli extract, reaction buffer, RNase-Free Water, and Positive-control DNA
32501
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EasyXpress Protein Synthesis Kit (20)
For 20 x 50 µl reactions: E. coli extract, reaction buffer, RNase-Free Water, and Positive-control DNA
32502
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EasyXpress Protein Synthesis Maxi Kit
For reactions up to 4000 µl: 4 x 350 µl E. coli extract, reaction buffer, RNase-Free Water, and Positive-control DNA
32506
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EasyXpress Protein Synthesis Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.


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High yields on any reaction scale.|
Using the EasyXpress Protein Synthesis Kit, EF-Ts protein was expressed in translation reactions in vitro performed in the indicated reaction volume for 1 hour.  Coomassie-stained SDS-PAGE gel. C: No template control reaction; M: markers.|
Performance
The EasyXpress Protein Synthesis Kit enables synthesis of active, full-length recombinant proteins directly from plasmid DNA or PCR product templates. Using optimized cell-free E. coli extracts, proteins are produced in high yields (see figure "High yields on any reaction scale"), high purity (see figure "Efficient synthesis and purification of active protein (Coomasie stain)" and are fully functional "Efficient synthesis and purification of active protein (silver stain)".
Principle

The EasyXpress Protein Synthesis Kit enables synthesis of active, full-length recombinant proteins directly from plasmid DNA or PCR product templates.

In combination with the EasyXpress Linear Template Kit the complete protein synthesis procedure, from template generation to protein purification, can be performed in a single day.

Applications

Proteins produced using the EasyXpress Protein Synthesis system are suitable for use in a wide variety of downstream applications, including activity assays, protein–protein interaction studies, structural and mutational analyses, and antibody generation.

Feature
Specifications
Applications In vitro expression of recombinant proteins
Expression In vitro
Expression in prokaryotic or eukaryotic cells Expression in cell-free E. coli expression systems
Expression vector/template Template
Scale Variable
Yield up to 50 µg/reaction

Kit Handbooks
1
For scalable cell-free expression of recombinant
proteins using E. coli lysates
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