For purification of up to 500 µg transfection-grade plasmid or cosmid DNA
QIAGEN Plasmid Kits provide gravity-flow, anion-exchange tips for purification of transfection-grade plasmid DNA. The QIAGEN Plasmid Maxi Kit delivers DNA yields of up to 500 μg. Lysate clearing and isopropanol precipitation are achieved by centrifugation.
The unique anion-exchange resin in QIAGEN-tips is developed exclusively for the purification of nucleic acids. Its exceptional separation properties result in DNA purity equivalent or superior to that obtained by two successive rounds of CsCl gradient centrifugation. Prepacked QIAGEN-tips operate by gravity flow and never run dry, minimizing the hands-on time required for plasmid preparation. The entire QIAGEN plasmid purification system avoids the use of toxic substances such as phenol, chloroform, ethidium bromide, and CsCl, minimizing hazard both to the user and the environment.
Plasmid DNA purified with QIAGEN Plasmid Kits is highly suited for use in applications, such as: