For fast purification of up to 500 µg transfection-grade plasmid or cosmid DNA

  • Lysate clearing without centrifugation
  • Reduced plasmid purification time
  • High yields of plasmid DNA
  • LyseBlue reagent for optimum lysis and maximum DNA yield

QIAfilter Plasmid Kits provide anion-exchange-based plasmid DNA purification with bacterial lysate clearing by filtration. The kits deliver DNA yields of up to 500 μg. The purified DNA is equivalent to that obtained by 2 x CsCl gradient centrifugation and is suitable for transfection-grade applications.

Quantity Product Cat. no. Price
 
 
show details
  varies
Quantity Product Cat. no. Price Sum
 
QIAfilter Plasmid Maxi Kit (10)
10 QIAGEN-tip 500, Reagents, Buffers, 10 QIAfilter Maxi Cartridges
12262
$255.00
$0.00
 
QIAfilter Plasmid Maxi Kit (25)
25 QIAGEN-tip 500, Reagents, Buffers, 25 QIAfilter Maxi Cartridges
12263
$619.00
$0.00
The QIAfilter Plasmid Maxi Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Comparison of QIAGEN Plasmid Kit procedures.|
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Performance

The QIAfilter Plasmid Maxi Kit combines QIAfilter Maxi cartridges for rapid clearing of bacterial lysates by filtration instead of centrifugation, with proven QIAGEN-tip 500 anion-exchange tips for efficient plasmid purification.  Using the syringe-format QIAfilter Maxi Cartridge and the QIAGEN-tip 500, up to 500 µg transfection-grade plasmid DNA can be purified from 100–250 ml culture. (Culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium.) 

Principle

QIAfilter Cartridges, provided in QIAfilter, HiSpeed, and EndoFree Plasmid Kits, are special filter units designed to replace centrifugation following alkaline lysis of bacterial cells. QIAfilter Cartridges completely remove SDS precipitates and clear bacterial lysates in a fraction of the time needed for centrifugation, reducing plasmid-purification time by up to 1 hour.

QIAfilter Maxi Cartridges have a syringe-format and lysates are cleared rapidly by pushing the liquid through the filter.

The unique anion-exchange resin in QIAGEN-tips is developed exclusively for the purification of nucleic acids. Its exceptional separation properties result in DNA purity equivalent to that obtained by two successive rounds of CsCl gradient centrifugation. Prepacked QIAGEN-tips operate by gravity flow and never run dry, minimizing the hands-on time required for plasmid preparation. The entire QIAGEN plasmid purification system avoids the use of toxic substances such as phenol, chloroform, ethidium bromide, and CsCl, minimizing hazard both to the user and the environment.

Procedure

Neutralized bacterial lysates are incubated directly in the QIAfilter Cartridge and cleared rapidly by filtration. The cleared lysate is then loaded onto the anion-exchange tip where plasmid DNA selectively binds under appropriate low-salt and pH conditions. RNA, proteins, metabolites, and other low-molecular-weight impurities are removed by a medium-salt wash, and ultrapure plasmid DNA is eluted in high-salt buffer (see flowchart "QIAGEN Plasmid Kit procedures"). The DNA is concentrated and desalted by isopropanol precipitation and collected by centrifugation.

Applications

DNA obtained with QIAfilter Plasmid Kits provides excellent results in all applications, from cloning to transfection.

Feature
Specifications
Applications Transfection, cloning, sequencing etc.
Culture volume/starting material 100–250 ml culture volume
Plasmid type High-copy, low-copy, cosmid DNA
Processing Manual (filtration)
Samples per run; throughput 1 sample per run
Technology Anion-exchange technology
Time per run or prep per run 120 min
Yield <500 µg

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