For blood collection prior to genomic DNA purification using the PAXgene Blood DNA Kit
  • Storage for up to 14 days at room temperature
  • Easy handling
  • Blood collection and purification in one system
  • Enhanced workflow efficiency

The PAXgene Blood DNA system consists of PAXgene Blood DNA Tubes, for blood collection and stabilization, and the PAXgene Blood DNA Kit, for DNA purification in a single-tube procedure.

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PAXgene Blood DNA Tubes (100)
100 PAXgene Blood DNA Tubes
761115
$139.00
$0.00
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
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PAXgene Blood DNA procedure.|Efficient multiplex PCR of 3 mitochondrial genes.|High quality and high molecular weight of genomic DNA.|
Blood samples (8.5 ml) are collected in PAXgene Blood DNA Tubes, where they may be stored or transported. For DNA isolation, the blood is transferred to processing tubes (supplied already filled with cell lysis buffer), and the solution is mixed to lyse red and white blood cells. Cell nuclei and mitochondria are pelleted by centrifugation, washed, and resuspended in digestion buffer. Protein contaminants are removed by incubation with a protease. DNA is precipitated in isopropanol, washed in 70% ethanol, dried, and resuspended in resuspension buffer.|Multiplex PCR of fragments from the mitochondrial genes tRNAlys/ATPase (0.92 kb), tRNAleu(UUR) (0.63 kb), and ND4 (0.29 kb), using 250 ng DNA from 8 donors as starting material.|Genomic DNA isolated from 8 blood donors using the PAXgene Blood DNA System. [A] Agarose gel analysis; [B] pulsed-field gel electrophoresis for enhanced separation of high-molecular-weight genomic DNA.  M: markers.|
Performance
DNA isolated using the PAXgene Blood DNA System has an A260/A280 ratio of 1.7–1.9. Fragments are 20–200 kb in size, with an average length of 50–150 kb (see figure "High quality and high molecular weight of genomic DNA"). Average DNA yields are 150–500 µg, depending on the number of nucleated cells present in the blood sample. Purified DNA can be used for PCR and quantitative, real-time PCR (see figure "Efficient multiplex PCR of 3 mitochondrial genes").
Principle

The PAXgene Blood DNA System is an integrated and standardized system for collection and stabilization of whole blood specimens and isolation of their genomic DNA. The system requires the combined use of PAXgene Blood DNA Tubes for blood collection and stabilization, followed by DNA isolation using the PAXgene Blood DNA Kit.

Blood is collected into PAXgene Blood DNA Tubes, which contain a proprietary blend of reagents that both prevents blood coagulation and stabilizes white blood cells. Blood samples can be stored in the tubes for up to 14 days at room temperature. DNA is isolated from the tubes using appropriate buffers supplied in the PAXgene Blood DNA Kit. Highly pure genomic DNA is obtained, suitable for use in a wide range of downstream applications.

Procedure

The PAXgene Blood DNA procedure is shown in the flowchart "PAXgene Blood DNA procedure". Blood samples (8.5 ml) are collected in PAXgene Blood DNA Tubes, where they may be stored or transported. For DNA isolation, the blood is transferred to processing tubes (supplied already filled with cell lysis buffer), and the solution is mixed to lyse red and white blood cells. Cell nuclei and mitochondria are pelleted by centrifugation, washed, and resuspended in digestion buffer. Protein contaminants are removed by incubation with a protease. DNA is precipitated in isopropanol, washed in 70% ethanol, dried, and resuspended in resuspension buffer.

Applications

The DNA can be used in a wide range of downstream applications, including:

PCR and quantitative, real-time PCR
Southern blotting
SNP genotyping
Pharmacogenomic studies
SNP discovery and validation
Feature
Specifications
Tube size 16 x 100 mm
Draw volume 8.5 ml
Closure type/color BD Hemogard/Blue
Label type Paper
Additive/concentration Additive 2 ml
Quantity 100/box