For purification of archive-quality DNA from cell cultures and cell suspensions
  • Archive-quality DNA for long-term storage
  • Reproducible purification from a range of sample types
  • A complete solution for sample-to-storage purification
  • Convenient, scalable purification procedure

The Gentra Puregene Cell Kit enables purification of high-molecular-weight (100–200 kb) DNA suitable for archiving. The scalable purification procedure gently removes contaminants and inhibitors and allows samples to be purified for use as long-term references.

Quantity Product Cat. no. Price
 
 
show details
  varies Add to wishlist
Please note: 158722: Gentra Puregene Cell Kit (2 x 107) has been discontinued as of December 31, 2013. We recommend the alternative 158745: Gentra Puregene Cell Kit (2 x 108). For more information, please contact QIAGEN Technical Services.
Quantity Product Cat. no. Price Sum
 
Gentra Puregene Cell Kit (2 x 107)
For (2 x 107) cells: RNase A Solution and Reagents
158722
Inquire
 
Gentra Puregene Cell Kit (2 x 108)
For (2 x 108) cells: RNase A Solution and Reagents
158745
$104.00
$0.00
 
Gentra Puregene Cell Kit (8 x 108)
For (8 x 108) cells: RNase A Solution and Reagents
158767
$245.00
$0.00
 
Gentra Puregene Cell Kit (6.7 x 109)
For (6.7 x 109) cells: Reagents
158388
$920.00
$0.00
 
Gentra Puregene Cell Kit Plus (6.7 x 109)
For (6.7 x 109) cells: RNase A Solution and Reagents
158788
$1,156.00
$0.00
The Gentra Puregene Cell Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
  • Main Image Navi
Puregene DNA procedure.|Reproducible purification of archive-quality DNA.|
The simple Puregene procedure uses a modified salting-out precipitation method for purification of DNA. No mixing or dilution of solutions is necessary, and hands-on time is minimized. The procedure provides convenient stopping points that allow safe, temporary storage of partially purified samples.|DNA was purified from 8 replicate IM9 cultured cell samples (1.5 x 108 cells each) using the Gentra Puregene Cell Kit. Purified DNA was analyzed by agarose gel electrophoresis. M: Lambda HindIII marker.|
Performance
Archive-quality DNA 

Purity of DNA has a significant effect on the accuracy of results obtained in downstream applications. Sensitive downstream applications, such as PCR, demand the use of DNA of the highest quality and molecular weight for reliable results. The Gentra Puregene Cell Kit removes contaminants and enzyme inhibitors enabling purification of highly stable DNA well-suited for archiving. Purified DNA is of high quality demonstrated by molecular weights of 100–200 kb (see figure "Reproducible purification of archive-quality DNA").

Principle
Cells are lysed with an anionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. Finally, the genomic DNA is recovered by precipitation with alcohol and dissolved in hydration solution (1 mM EDTA, 10 mM Tris·Cl pH 7.5). Purified DNA typically has an A260/A280 ratio between 1.7 and 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2–8°C, –20°C, or –80°C.
Procedure
The simple Puregene procedure uses a modified salting-out precipitation method for purification of DNA (see flowchart "Puregene DNA procedure"). No mixing or dilution of solutions is necessary, and hands-on time is minimized. The procedure provides convenient stopping points that allow safe, temporary storage of partially purified samples.
Applications

DNA purified using the Gentra Puregene Cell Kit is highly stable and suited for use in a wide range of applications, such as:

DNA archiving
PCR
SNP analysis
Southern blotting
Feature
Specifications
Applications PCR, restriction digest, Southern analysis, SNP analysis
Elution volume 50 - 250 µl (varies)
Format Scalable
Main sample type Cultured cells
Processing Manual
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Genomic DNA
Sample amount 10e6 - 2.2*10e7
Technology Modified salting-out precipitation method
Time per run or per prep 25 - 60 minutes (+ DNA rehydration)
Yield 7 µg / 1 million cells

Supplementary Protocols
14
This protocol is designed for purification of DNA from 100-150 mg dried or 200-400 mg fresh leaf tissue using the Gentra Puregene Cell Kit.
Show details
This protocol is designed for purification of DNA from 600-1200 mg fresh or frozen leaf tissue or 300-450 mg dried leaf tissue using the Gentra Puregene Cell Kit.
Show details
This protocol is designed for purification of DNA from 25 ml Gram-negative bacteria culture medium using the Gentra Puregene Cell Kit.
Show details
This protocol is designed for purification of DNA from 1, 10-15, or 20-30 Drosophila melanogaster using the Gentra Puregene Cell Kit.
Show details
This protocol provides information about scaling of reagents required for purification of DNA from 100 to 5 x 108 cultured cells using the Gentra Puregene Cell Kit.
Show details
This protocol is designed for purification of DNA from 1 g samples of soil using the Gentra Puregene Cell Kit.
Show details
This protocol is designed for purification of DNA from 100 Drosophila melanogaster using the Gentra Puregene Cell Kit.
Show details
This protocol is designed for purification of DNA from 50-75 mg dried or 100-200 mg fresh leaf tissue using the Gentra Puregene Cell Kit.
Show details
This protocol is designed for purification of DNA from 5 ml Gram-negative bacteria culture medium using the Gentra Puregene Cell Kit.
Show details
This protocol is designed for purification of DNA from 0.5 ml or 1 ml Gram-negative bacteria culture medium using the Gentra Puregene Cell Kit.
Show details
This protocol is designed for purification of DNA from 300-700 (300-700 mg) Drosophila melanogaster using the Gentra Puregene Cell Kit.
Show details
This protocol is designed for rapid purification of DNA from 1-2 x 106, 3-5 x 106, or 6-9 x 106 cells using the Gentra Puregene Cell Kit.
Show details
This protocol is designed for purification of DNA from blood smears on a slide using Gentra Puregene Cell Kits.
Show details
This protocol is designed for purification of DNA from 0.5-2 mg samples of Eimeria oocysts using Gentra Puregene Cell Kits.
Show details
FAQs
13
FAQ ID -1969
View
FAQ ID -2809
View
FAQ ID -1960
View
FAQ ID -2812
View

View
FAQ ID -1975
View
FAQ ID -754
View
FAQ ID -2813
View
FAQ ID -1952
View
FAQ ID -1948
View
FAQ ID -618
View
FAQ ID -728
View
FAQ ID -2810
View
Kit Handbooks
2
以下サンプルから長期保存に安定なゲノムDNAの精製: ヒト全血; 骨髄液 ; バフィーコート; 口腔細胞; 体液; 培養細胞; 組織; マウス尾; 酵母; バクテリア
Show details
For purification of archive-quality DNA from human whole blood, bone marrow, buffy coat, buccal cells, body fluids, cultured cells, tissue, mouse tail, yeast, bacteria
Show details