For gel extraction/cleanup of up to 10 μg DNA (70 bp to 10 kb) from enzymatic reactions

  • Up to 95% recovery of ready-to-use DNA
  • Fast and convenient procedure
  • Cleanup of DNA up to 10 kb in three easy steps
  • Gel loading dye for convenient sample analysis

The QIAquick Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments from gels (up to 400 mg slices) or enzymatic reactions. DNA ranging from 70 bp to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 μl. An integrated pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. The procedure can be fully automated on the QIAcube.

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Quantity Product Cat. no. Price Sum
 
QIAquick Gel Extraction Kit (50)
For gel extraction or cleanup of 50 reactions: 50 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)
28704
$111.00
$0.00
 
QIAquick Gel Extraction Kit (250)
For gel extraction or cleanup of 250 reactions: 250 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)
28706
$527.00
$0.00
The QIAquick Gel Extraction Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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QIAquick and MinElute procedure.|High recoveries from gels.|GelPilot Loading Dye.|pH Indicator Dye.|Spin column handling options — D.|Sin column handling options — E.|Spin column handling options — C.|Spin column handling options — B.|Spin column handling options — A.|Reliable sequencing after gel extraction.|
The QIAquick and MinElute systems use a simple bind-wash-elute procedure with spin columns or a vacuum manifold.
|DNA fragments (sizes indicated) before extraction with the QIAquick Gel Extraction Kit and pooled after extraction are shown. Recoveries of approximately 80% are obtained for all fragment sizes [A] 1–5: before extraction; P: pooled after extraction. Samples were analyzed on a 1.5% agarose gel in TAE buffer. [B] 1–3: before extraction; P: pooled after extraction. Samples were analyzed on a 3.5% high-resolution agarose gel in TAE buffer. M: pTZ-HinfI markers.|GelPilot Loading Dye contains three tracking dyes to facilitate optimization of DNA resolution.

|pH indicator dye in the solubilization and binding buffer allows easy visual determination of optimal pH for DNA adsorption (pH ≤7.5). An incorrect binding-mixture pH can occur if the agarose gel electrophoresis buffer was frequently used or incorrectly prepared. In this case, the pH can be easily adjusted by addition of 10 µl 3 M sodium acetate, pH 5.0.
|QIAvac 24 plus.
|QIAcube.
|Manifold with luer connectors.
|QIAvac 24.
|Microcentrifuge.
|Sequence of a 2.7 kb PCR product extracted from a TAE agarose gel with the QIAquick Gel Extraction Kit is shown.
|
Performance
The QIAquick Gel Extraction Kit enables removal of nucleotides, enzymes, salts, agarose, ethidium bromide, and other impurities from samples, ensuring up to 80% recovery of DNA (see figure "High recoveries from gels"). Using a microcentrifuge or vacuum manifold, DNA ranging from 70 bp to 10 kb is purified from 1–24 samples. Purified DNA can be used, for example, in sequencing (see figure "Reliable sequencing after gel extraction"). DNA fragments smaller than 70 bp or larger than 10 kb should be extracted with the QIAEX II Gel Extraction System.
Principle

QIAquick Kits contain a silica membrane assembly for binding of DNA in high-salt buffer and elution with low-salt buffer or water. The purification procedure removes primers, nucleotides, enzymes, mineral oil, salts, agarose, ethidium bromide, and other impurities from DNA samples (see figure "High recoveries from gels"). Silica-membrane technology eliminates the problems and inconvenience associated with loose resins and slurries. Specialized binding buffers are optimized for specific applications and promote selective adsorption of DNA molecules within particular size ranges.

Gel loading dye

To enable faster and more convenient sample processing and analysis, gel loading dye is provided. GelPilot Loading Dye contains three tracking dyes (xylene cyanol, bromophenol blue, and orange G) to facilitate the optimization of agarose gel run time and prevent smaller DNA fragments migrating too far (see figure "GelPilot Loading Dye").

Procedure
The QIAquick system uses a simple bind-wash-elute procedure (see flowchart "QIAquick and MinElute procedure"). Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the QIAquick spin column (see figure "pH Indicator Dye"). Nucleic acids adsorb to the silica membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in all subsequent applications.
Handling

QIAquick spin columns are designed to provide two convenient handling options. The spin columns fit into a conventional table-top microcentrifuge or onto any vacuum manifold with luer connectors, such as the QIAvac 24 Plus with QIAvac Luer Adapters. The QIAquick Gel Extraction Kit, in addition to other QIAGEN spin-column-based kits, can be fully automated on the QIAcube, enabling increased productivity and standardization of results (see figures "Spin column handling options A, B, C, D, and E").

Applications

DNA fragments purified with the QIAquick system are ready for direct use in all applications, including sequencing, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription.

Feature
Specifications
Binding capacity 10 µg
Elution volume 30–50 µl
Format Tube
Fragment size 70 bp – 10 kb
Processing Manual
Recovery: oligonucleotides dsDNA Recovery: dsDNA fragments
Removal <10mers 17–40mers dye terminator proteins Removal <10mers
Sample type: applications DNA: PCR reactions
Technology Silica technology

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Kit Handbooks
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