For safer and convenient extraction of nucleic acids from organic solvents
  • Safer extraction of nucleic acids from organic solvents
  • Reduced carryover of contaminants
  • High nucleic acid recovery
  • Convenient nucleic acid recovery

MaXtract High Density simplifies the extraction of nucleic acids from organic solvents (e.g., phenol/chloroform). The MaXtract gel forms a stable barrier between the organic solvent and the nucleic acid-containing aqueous phase allowing easy recovery of the aqueous phase while preventing carryover of organic solutions, proteins and other contaminants.

Quantity Product Cat. no. Price
 
 
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  varies
Quantity Product Cat. no. Price Sum
 
MaXtract High Density (200 x 1.5 ml)
200 x 1.5 ml MaXtract High Density Tubes
129046
$181.00
$0.00
 
MaXtract High Density (200 x 2 ml)
200 x 2 ml MaXtract High Density Tubes
129056
$184.00
$0.00
 
MaXtract High Density (100 x 15 ml)
100 x 15 ml MaXtract High Density Tubes
129065
$156.00
$0.00
 
MaXtract High Density (25 x 50 ml)
25 x 50 ml MaXtract High Density Tubes
129073
$138.00
$0.00
The MaXtract High Density is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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MaXtract procedure.|Safe and easy nucleic acid extraction.|
The MaXtract procedure affords reliability and convenience.|MaXtract gel forms a safe and stable barrier between the organic solvent and the nucleic acid-containing aqueous phase, enabling simple separation by pouring.|
Performance

MaXtract High Density enables 30% higher recovery compared to traditional extraction methods. Proteins and other contaminants migrate to the organic phase and interphase. The barrier formed by MaXtract High Density gel is sufficiently durable that these contaminants remain trapped below the barrier, preventing carryover when removing the aqueous phase. The aqueous phase can be removed by decanting or pipetting, leading to nucleic acid recoveries that are up to 30% higher than when using traditional organic extraction methods.

MaXtract High Density is provided in a variety of tube sizes, enabling extraction from sample volumes ranging from 100 µl to 20 ml.

Phase separation by MaXtract High Density
Aqueous phase Phenol:chloroform Chloroform
<0.5 M NaCl, <1 mg/ml protein Yes Yes
≥0.5 M NaCl Yes Yes
≥1 mg/ml protein Yes Yes
Plasmid DNA isolation Yes Yes
Genomic DNA isolation Yes Yes
RNA isolation Yes Yes
* Includes phenol:chloroform:isoamyl alcohol.
Principle
MaXtract High Density gel provides safe and fast recovery of nucleic acids from organic extractions using solvents such as phenol/chloroform (see flowchart "MaXtract procedure").
Procedure

Simply add the nucleic acid solution and organic solvent to the tube containing MaXtract gel. After mixing and centrifugation, MaXtract High Density gel forms a stable barrier, separating the organic and aqueous phases. The barrier safely traps the hazardous organic solvent and contaminants, allowing you to easily decant or pipet the nucleic-acid–containing aqueous phase into a new tube (see figure "Safe and easy nucleic acid extraction"). If a second extraction is necessary, this can be performed in the same tube, provided that the maximum tube volume is not exceeded.

Separation using MaXtract High Density gel relies on density differences between the aqueous and organic media. The organic layer must have a higher density than the gel and the aqueous phase, and the gel must have a higher density than the aqueous phase.

Applications
MaXtract High Density is highly suited for isolation of plasmid DNA, genomic DNA, or RNA. Protocols optimized for specific applications, such as recovery of DNA from low-melting-point agarose, are also available. Nucleic acids extracted using MaXtract High Density gel are suitable for many downstream applications.

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