For fully integrated automation of complete workflows, from sample preparation to assay setup
  • Innovative, easy-to-use modular system with built-in touchscreen
  • Purification of DNA and RNA from a wide range of samples
  • Automatic transfer of eluates to the QIAsymphony AS for assay setup
  • Continuous sample loading, with bar code reading for sample tracking
  • Import of sample lists and export of sample sheets

With a dedicated range of QIAsymphony Kits, the QIAsymphony SP enables sample preparation of DNA, RNA, and bacterial and viral nucleic acids from a wide range of starting materials. The QIAsymphony AS extends the capabilities of the QIAsymphony SP by integrating automated PCR assay setup, which, in combination with the Rotor-Gene Q and QIAGEN real-time and end-point PCR kits, enables you to optimize your PCR workflow and maximize your efficiency.

Quantity Product Cat. no. Price
 
 
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Quantity Product Cat. no. Price Sum
 
QIAsymphony SP
QIAsymphony sample prep module: includes 1-year warranty on parts and labor
9001297
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QIAsymphony SP System
QIAsymphony sample prep module: includes installation and training, 1-year warranty on parts and labor
9001751
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QIAsymphony SP Priority Package
QIAsymphony sample prep module: includes Priority Package with software, installation, training, 2-year warranty on parts and labor, and 2 preventive maintenance visits
9001880
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QIAsymphony SP Priority Package Plus
QIAsymphony sample prep module: includes Priority Package with software, installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits
9001881
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QIAsymphony AS
QIAsymphony assay setup module: includes 1-year warranty on parts and labor
9001301
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QIAsymphony Cabinet SP/AS
Accessory for correct positioning of the QIAsymphony SP/AS instruments
9020246
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QIAsymphony Cabinet AS
Accessory for correct positioning of the QIAsymphony AS instrument
9020245
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QIAsymphony Cabinet SP
Accessory for correct positioning of the QIAsymphony SP instrument
9020244
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The QIAsymphony SP is intended to be used only in combination with QIAsymphony Kits indicated for use with the QIAsymphony SP for the applications described in the kit handbooks. If used in combination with QIAGEN Kits indicated for use with the QIAsymhony AS instrument, the QIAsymphony AS is intended for the applications described in the respective QIAGEN Kit handbooks. If the QIAsymphony AS instrument is used with kits other than QIAGEN Kits, it is the user’s responsibility to validate the performance of such product combination for any particular application.


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QIAsymphony workflows.|Prefilled, sealed reagent cartridges.|QIAsymphony Cabinet SP/AS.|
The QIAsymphony SP/AS instruments can be operated in independent or integrated mode.|Reagent cartridges are prefilled with all reagents required for the purification procedure, including accessory enzymes, and are labeled with 2D bar codes. Partially used cartridges can be resealed for later use, giving you complete cost control.|The QIAsymphony Cabinet SP and the QIAsymphony Cabinet AS are designed for correct positioning of the QIAsymphony SP/AS instruments.|
Principle

The QIAsymphony SP can save you time by processing 1–96 samples — in batches of 24 — with sample volumes up to 2 ml. A dedicated range of QIAsymphony Kits increase laboratory efficiency since just a few kits in combination with optimized protocols cover an extensive range of starting materials and applications. QIAsymphony technology combines the speed and efficiency of silica-based purification with the convenient handling of magnetic particles.

Dedicated QIAsymphony Kits for a wide range of starting materials
Kits Starting material and amount
QIAsymphony DNA Kit Purification of DNA from up to 1000 µl human whole blood, 400 µl buffy coat, 50 mg tissue (including FFPE tissue), or 1 x 107 cells, and purification of viral nucleic acids from blood
QIAsymphony DSP DNA Kit Purification of DNA from up to 1000 µl human whole blood, 400 µl buffy coat, tissue (including FFPE tissue), bacterial and cell cultures, and purification of viral nucleic acids from blood for in vitro diagnostic use
QIAsymphony DNA Investigator Kit Purification of reference and casework samples for human identity testing and forensics
QIAsymphony DSP Virus/Pathogen Kit  Purification of viral nucleic acids from plasma, serum, and CSF, and viral nucleic acids and bacterial DNA from respiratory and urogenital samples for in vitro diagnostic use
QIAsymphony DSP AXpH DNA Kit Purification of DNA from liquid-based cytology samples using AXpH technology, for in vitro diagnostic use
QIAsymphony RNA Kit Purification of total RNA and miRNA from up to 50 mg tissue (including FFPE tissue) or 1 x 107 cells
QIAsymphony PAXgene Blood RNA Kit* Purification of intracellular RNA (including miRNA) from 2.5 ml human whole blood collected in PAXgene Blood RNA Tubes
QIAsymphony mericon Bacteria Kit Purification of bacterial DNA from 500 μl enrichment culture
QIAsymphony Certal Kits Purification of residual host cell DNA and viral NA from bioprocess purification buffer, cell culture supernatant samples, and vaccine preparations
* Up to 72 samples can be processed per run.
  

Extending the QIAsymphony SP with the QIAsymphony AS results in a fully integrated system that can automate complete sample preparation and assay setup workflows. Manual pipetting steps prone to human error are eliminated, making this system ideal for laboratories that perform complex PCR assays on a day-to-day basis. The QIAsymphony AS provides active cooling of reagents, eluates, and assays, enabling safe and reproducible assay setup. The QIAsymphony AS enables setup of multiple assays per run or sample and supports QIAGEN PCR and RT-PCR kits. A customization service is available for in-house assays.

With innovative design features, the QIAsymphony SP/AS instruments deliver exceptional process safety and standardization. Tip guards minimize the risk of cross-contamination and built-in UV lamps provide effective worktable decontamination. Bar code reading of samples and reagents enables full sample tracking throughout the entire purification and assay setup procedure. Chronological electronic documentation of results is provided, including information about sample IDs, their positions, and the lot number and expiry date of sample preparation and PCR reagents. Multi-level user management provides a record of which user was responsible for sample processing. Electronic result files may be automatically printed via a networked printer, or downloaded using a USB stick for archiving purposes.

The QIAsymphony Cabinet SP/AS (see figure "QIAsymphony Cabinet SP/AS") is specifically designed for correct positioning of the QIAsymphony SP/AS instruments. The QIAsymphony Cabinet SP/AS comprises two separate parts: one for the QIAsymphony SP and one for the QIAsymphony AS. If you have QIAsymphony SP and QIAsymphony AS instruments, you need both cabinet parts, which are then positioned side by side. If you just have a QIAsymphony SP instrument, only the QIAsymphony Cabinet SP is required. The QIAsymphony Cabinet SP and the QIAsymphony Cabinet AS each contain a waste compartment into which used tips from the worktable can be ejected.

 

Procedure

The QIAsymphony SP is ready-to-run and requires only minimal installation. Laboratories already operating a QIAsymphony SP can easily upgrade their system onsite with a QIAsymphony AS module. The QIAsymphony AS directly interfaces with the QIAsymphony SP. To reduce manual handling steps and minimize the risk of sample contamination, samples processed on the QIAsymphony SP can be transferred automatically to the QIAsymphony AS (integrated operation). For extra flexibility, the QIAsymphony SP and AS can also be operated independently. Samples that have been processed earlier in the day, or that have been processed on a different QIAsymphony SP, may be manually transferred to the QIAsymphony AS (see figure "QIAsymphony workflows").

Sample preparation and assay setup runs are defined using the touchscreen. Simply load drawers with the required samples, reagents, and consumables. When each drawer is closed, a detailed inventory scan is performed. This ensures that the worktable is correctly set up for the selected protocols. After sample preparation, either remove purified nucleic acids from the eluate drawer or automatically transfer eluates to the QIAsymphony AS via the transfer module for assay setup. After assay setup, assays can be transferred directly to a real-time PCR cycler for analysis.

The sample input drawer accommodates a wide range of sample input formats to suit varying workflows and sample preparation requirements. Bar code-labeled primary and secondary sample tubes (diameter 10–16.9 mm), microplates, and blocks are supported. Continuous loading in batches of up to 24 samples plus internal controls, and the option to assign different protocols to each batch, delivers increased efficiency and walkaway time. The QIAsymphony AS enables setup of single or multiple PCRs per run or sample, splitting of eluates for panel testing, and active cooling of eluates, reagents, and PCRs. A choice of output adapters enables use of different real-time PCR cyclers (e.g., Rotor-Gene Q, 96-well cyclers, 32-capillary cyclers). To use the Rotor-Gene Q for subsequent detection, simply set up your PCRs in Strip Tubes or Rotor-Discs.

QIAsymphony Kits are safe and exceptionally easy to use. Ready-to-run, bar code-labeled reagent cartridges are prefilled with all reagents required for the purification procedure, including accessory enzymes (see figure "Prefilled, sealed reagent cartridges"). Worktable setup is rapid and saves you valuable time. Simply place up to 2 reagent cartridges in the QIAsymphony drawer. The reagent cartridges can be from the same or different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent cartridges are automatically opened by the instrument, and reagent volumes only for the selected number of samples are used, giving you complete cost control.

Applications

The QIAsymphony SP/AS instruments are suitable for laboratories performing applications, such as:

  • Molecular diagnostics research and biomedical research
  • Human identity testing
  • Veterinary applications
  • Biosecurity research
  • Life science applications, such as genomics research
Software

QIAsymphony operating software guides the user step-by-step through setting up a sample preparation or assay setup run, with options to select and adapt standardized protocols. Required volumes of buffers, reagents, master mix, and controls are automatically calculated, and the user is informed of the number and type of consumables to be loaded. Transfer of sample loading information from the QIAsymphony SP to the QIAsymphony AS is convenient and reduces the number of steps required for assay definition.

The QIAsymphony SP/AS instruments provide standardized communication interfaces. Work lists and rack files may be imported to the instruments, enabling automatic run definition before samples are received in the laboratory. After assay setup, sample lists can be exported to selected real-time PCR cyclers. This bi-directional data exchange allows integration of QIAsymphony SP/AS instruments into laboratory information management systems (LIMS).

Feature
Specifications
Altitude Up to 2000 m
Elution volume QIAsymphony SP, 50–400 µl (application/protocol dependent)
Humidity Relative humidity of 15–75% (noncondensing); maximum 75% relative humidity for temperatures up to 31°C, decreasing linearly to 50% humidity at 32°C
Input volume QIAsymphony SP, 100–2000 µl (application/protocol dependent)
Instrument dimensions QIAsymphony SP, 130 x 75 x 103 cm; QIAsymphony AS, 59 x 103 x 73 cm; QIAsymphony SP/AS (integrated operation), 185 x 103 x 73 cm
Kits or Application Packs designed for this instrument QIAsymphony SP — QIAsymphony DSP Virus/Pathogen Kits and other QIAsymphony Kits; QIAsymphony AS — artus QS-RGQ Kits (not available in all countries), Rotor-Gene Kits and other QIAGEN real-time and end-point PCR kits
Operating temperature 15–32°C
Overvoltage category II
Place of operation For indoor use only
Pollution level 2
Power 100–240 V AC, 50–60 Hz, 1400 VA, mains supply voltage are not to exceed 10% of nominal supply voltages
Processing Automated
Protocols/main application on this instrument QIAsymphony SP — Purification of DNA, RNA, and bacterial and viral nucleic acids from a wide range of starting materials; QIAsymphony AS — PCR setup.
Storage temperature 5–40°C in manufacturer's package
Technology QIAsymphony SP — QIAGEN magnetic-particles chemistry
Transportation temperature –25 to 70°C in manufacturer's package
Weight QIAsymphony SP, 175 kg; QIAsymphony AS, 90 kg; QIAsymphony SP/AS (integrated operation), 265 kg

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FAQs
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Application/Protocol Documents
27
For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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For use with the QIAsymphony AS (software version 4.0)
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Protocol Files
6
For use with the QIAsymphony SP/AS instruments (software version 4.0)
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For use with the QIAsymphony SP/AS instruments (software version 4.0)
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For use with the QIAsymphony SP/AS instruments (software version 4.0)
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For use with the QIAsymphony SP/AS instruments (software version 4.0)
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For use with the QIAsymphony SP/AS instruments (software version 4.0)
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For use with the QIAsymphony SP/AS instruments (software version 4.0)
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Operating Software
6
QIAsymphony Management Console operating software for QIAsymphony software version 4.0.2
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Operating software document for QIAsymphony software version 4.0.2
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Differences between QIAsymphony software versions
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Labware package SOW-516-E for use with the QIAsymphony SP/AS instruments (software version 4.0)
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Operating software for QIAsymphony software version 4.0.2
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Operating software for QIAsymphony software version 4.0.2
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Instrument User Manuals
5
For use with the QIAsymphony SP/AS instruments (software version 4.0)
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For use with the QIAsymphony SP/AS instruments (software version 4.0)
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Instrument Technical Documents
13
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0
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Script change document for software version 4.0
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QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0
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QIAsymphony Cabinet SP/AS documents
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Differences between QIAsymphony software versions
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QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0
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QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0
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Script change document for software version 4.0
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QIAsymphony Cabinet SP/AS document
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Script change document for software version 4.0
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Script change document for software version 4.0
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