For automated purification of viral nucleic acids from 1–6 or 1–14 samples per run
  • Simultaneous purification of viral DNA and RNA
  • Higher analytical sensitivity, even with low viral titers
  • Highly reproducible results
  • Linear yields from a wide range of viral titers
  • Prefilled, sealed reagent cartridges for easy handling

The EZ1 Virus Mini Kit v2.0 provides a fully automated procedure for simultaneous purification of viral DNA and RNA from serum, plasma, CSF, urine, respiratory samples, and other cell-free body fluids for sensitive analytical detection in downstream assays. The kit provides optimized binding conditions for more robust and reproducible capture of nucleic acids, and improved wash conditions for higher analytical sensitivity in downstream assays. The kit can be used with the EZ1 Advanced XL (1–14 samples per run), the EZ1 Advanced (1–6 samples per run), and the BioRobot EZ1 (1–6 samples per run). The EZ1 DSP Virus Kit is also available for viral nucleic acid purification for in vitro diagnostics (not available in all countries).

Quantity Product Cat. no. Price
 
 
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Quantity Product Cat. no. Price Sum
 
EZ1 Virus Mini Kit v2.0 (48)
For 48 preps: Reagent Cartridges (Virus Mini v2.0), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml), Buffer AVE, carrier RNA
955134
$357.00
$0.00
The EZ1 Virus Mini Kit v2.0 is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Principle

Automated purification with the EZ1 Virus Mini Kit v2.0 requires use of the EZ1 Advanced XL and the EZ1 Advanced XL Virus Card v2.0, or the EZ1 Advanced and the EZ1 Advanced Virus Card v2.0, or the BioRobot EZ1 and the EZ1 Virus Card v2.0. EZ1 cards are preprogrammed with protocols that guide the user through worktable setup and provide the instrument with commands for the purification procedure.

Using silica-coated magnetic-particle technology, the easy-to-use instrument allows purification of 1-14 samples (EZ1 Advanced XL) or 1-6 samples (EZ1 Advanced and BioRobot EZ1) in a single run. Presealed reagent cartridges and the automatic sensor-controlled lock of the instrument door reduce the chances of environmental contamination or degradation due to RNases.

Procedure

Serum or plasma samples (100 µl, 200 µl, or 400 µl) are loaded onto the instrument, and viral nucleic acids are purified in one step following the automated EZ1 Virus procedure. Viral nucleic acids are eluted in a volume of 150 µl, 120 µl, 90 µl, or 60 µl and are ready to use in downstream applications.

The instrument makes viral nucleic acid purification convenient and reliable. The EZ1 Virus Mini Kit v2.0 provides prefilled, sealed reagent cartridges that help to prevent human error during setup of the instrument, while the EZ1 Cards set the purification protocol without any manual data entry. Fully automated purification from 1–6 samples requires only 40 minutes, including only 5 minutes hands-on time.

Feature
Specifications
Applications PCR, real-time RT-PCR
Elution volume 50–200 µl
For automated processing BioRobot EZ1
Format Sample tube
Main sample type Serum, plasma
Processing Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Viral DNA, viral RNA
Sample amount 100 µl, 200 µl, 400 µl
Technology Magnetic particles
Time per run or per prep 40 minutes

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Kit Handbooks
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EZ1 Virus Mini Kit v2.0
For automated, simultaneous purification of viral DNA and RNA, and bacterial DNA, from 1-14 samples of serum, plasma, and CSF, urine, whole blood, and stool, transport media, respiratory samples, and dried swab samples using EZ1 instruments
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