For low-throughput sample disruption for molecular analysis
  • Rapid, effective disruption of a range of sample types
  • Disposable probes help to eliminate cross-contamination
  • Time savings through use of disposable probes
  • Visual monitoring of disruption using transparent probes
  • Seamless integration with QIAGEN sample technologies
The TissueRuptor is a handheld rotor-stator homogenizer that provides rapid, efficient, and flexible disruption of human, animal, and plant samples for a wide range of downstream applications. The TissueRuptor uses transparent disposable probes, which helps to minimize the risk of cross-contamination and enables visual control of the sample disruption process. Use of a disposable probe also provides time savings as the probe does not have to be cleaned after homogenization of each sample.
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Quantity Product Cat. no. Price Sum
 
TissueRuptor (120 V, 60 Hz, US/JP)
Handheld rotor–stator homogenizer, 120 V, 60 Hz (for North America and Japan), 5 TissueRuptor Disposable Probes
9001271
$878.00
$0.00
 
TissueRuptor Disposable Probes (25)
25 nonsterile plastic disposable probes for use with the TissueRuptor
990890
$302.00
$0.00
The TissueRuptor is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Reproducible purification of high-quality genomic DNA.|Efficient disruption and homogenization of animal tissues.|Successful purification of phosphoproteins.|High performance results in PCR.|Pure RNA with high RIN values.|
Frozen liver and kidney samples (30 mg each) were disrupted in lysis buffer at full speed for 30 seconds using the TissueRuptor. Genomic DNA was purified using the AllPrep DNA/RNA Mini Kit and analyzed by agarose gel electrophoresis. M: markers.|The following tissues were disrupted at full speed for 30 seconds using the TissueRuptor: frozen kidney, liver, and spleen (10 mg each); frozen lung (250 mg); stabilized heart, muscle, and brain (10 mg each). Samples were either disrupted in lysis buffer (kidney, liver, spleen, lung, heart, and muscle) or QIAzol Lysis Reagent (brain). Total RNA was purified using the RNeasy Plus Mini Kit (RNeasy Plus), the RNeasy Midi Kit (RNeasy Midi), the RNeasy Fibrous Tissue Mini Kit (RNeasy Fibrous), or the RNeasy Lipid Tissue Mini Kit (RNeasy Lipid). Purified RNA was analyzed by formaldehyde agarose gel electrophoresis. The gels show the high yields and quality of the RNA following disruption using the TissueRuptor.|Frozen rat liver and brain (approximately 30 mg each) were disrupted at medium speed for 30–60 seconds in 350 μl PhosphoProtein Lysis Buffer. The homogenized samples were then diluted with 1500 μl PhosphoProtein Lysis Buffer and incubated at 4°C for 30 minutes, with brief vortexing every 10 minutes. Phosphoproteins were purified using the PhosphoProtein Purification Kit and detected by western blotting. [A] Transfer membrane stained with Ponceau S, and [B] western blot. M: markers; FT: flow-through; E3: elution fraction 3.|[A] Liver and [B] heart samples (25 mg each, frozen) were disrupted with the TissueRuptor or a traditional rotor–stator homogenizer and lysed for 1 hour with proteinase K, or lysed overnight with proteinase K without sample disruption. DNA was purified using the QIAamp DNA Mini Kit. Eluates (5 μl) were subjected to PCR using HotStarTaq DNA Polymerase and primers specific for the 18S ribosomal RNA gene. M: GelPilot Mid Range Ladder.|Frozen liver samples (30 mg each) were disrupted at full speed for 30 seconds using either the TissueRuptor with disposable probes or a traditional rotor–stator homogenizer with a steel generator probe. Total RNA was purified using the RNeasy Plus Mini Kit and analyzed on the Agilent 2100 Bioanalyzer. The high RNA Integrity Number (RIN) indicates the high quality of the RNA.|
Performance
QIAGEN has tested use of the TissueRuptor in combination with proven QIAGEN sample preparation kits. Sample disruption with the TissueRuptor ensures maximal yields of nucleic acids and proteins that perform well in downstream applications such as PCR, western blotting, and enzyme assays (see figures "Successful purification of phosphoprotein " and "High performance results in PCR"). For most tissues, disruption and homogenization using the TissueRuptor gives comparable results to traditional rotor–stator homogenization (see figure "Pure RNA with high RIN values"). Effective disruption of tissue samples using the TissueRuptor allows reproducible purification of high-quality DNA and RNA using QIAGEN nucleic acid purification kits (see figures "Pure RNA with high RIN values", "Reproducible purification of high-quality genomic DNA", and "Efficient disruption and homogenization of animal tissues").
Principle

Genotyping, gene expression, and proteomics applications demand effective disruption of biological samples to ensure high yields of DNA, RNA, and protein. The TissueRuptor system delivers thorough and rapid disruption of samples to fully release biomolecules, and also simultaneously homogenizes samples to facilitate subsequent purification procedures using QIAGEN kits (see table).

The TissueRuptor is an integral part of QIAGEN's complete solution for sample management — from sample collection to purification and analysis of DNA, RNA, and protein. Optimized protocols integrate sample disruption with biomolecule purification, enabling a streamlined, efficient workflow. In addition, a range of automated solutions allow purification and analysis of biomolecules at low to high throughputs.

QIAGEN also provides RNAlater RNA Stabilization Reagent (to stabilize RNA) and Allprotect Tissue Reagent (to stabilize DNA, RNA, and protein). Tissues collected in these reagents can also be easily disrupted with the TissueRuptor.

QIAGEN purification kits compatible with QIAGEN disruption systems
Analyte purified Sample typeQIAGEN kit
RNA Easy-to-lyse tissue

RNeasy Kits

RNeasy Plus Kits
RNeasy Protect Kits
RNA Fiber-rich tissue RNeasy Fibrous Tissue Kits
RNA All types of tissue RNeasy Lipid Tissue Kits
RNeasy Universal Tissue Kits
RNA Plant tissue RNeasy Plant Mini Kit
RNA Yeast RNeasy Kits
RNA Bacteria RNeasy Protect Bacteria Kits
microRNA All types of tissue miRNeasy Kits
DNA Human tissue QIAamp DNA Kits
DNA Animal tissue DNeasy Blood & Tissue Kits
Protein Tissue Qproteome Mammalian Protein Prep Kit
Phosphoprotein Tissue PhosphoProtein Purification Kit
Glycoprotein Tissue Qproteome Glycoprotein Kits
DNA and RNA Tissue AllPrep DNA/RNA Kits
DNA, RNA, and protein Tissue AllPrep DNA/RNA/Protein Mini Kit

Procedure

The TissueRuptor is a portable device with a TissueRuptor Disposable Probe. The blade of the probe rotates at a high speed, causing simultaneous disruption and homogenization of a sample through a combination of turbulence and mechanical shearing. Samples that can be processed include human, animal, and plant tissues (for plant tissues that are more difficult to disrupt, the TissueLyser II is recommended). Disruption at full speed for as little as 30 seconds is usually sufficient to release nucleic acids or proteins from starting material.

After disruption of a sample, the TissueRuptor Disposable Probe can be discarded, and a new probe can be used to disrupt the next sample. Time is saved and cross-contamination is avoided, as there is no need to clean and reuse the same probe. In addition, the TissueRuptor Disposable Probe is transparent, which allows visual monitoring of the sample disruption process.

Applications

The TissueRuptor enables efficient disruption and homogenization of human, plant, and animal samples, including clotted blood. Purification of RNA, DNA, total nucleic acids, or protein can then be performed using QIAGEN kits.

Feature
Specifications
Disruption principle Simultaneous disruption and homogenization by rotation of the blade of the disposable probe
Features Rapid, effective disruption of a range of sample types. Disposable probes minimize risk of cross-contamination. Time savings through use of disposable probes. Visual monitoring of disruption using transparent probes. Seamless integration with QIAGEN sample technologies.
Kits compatible with instrument All Kits for purification of RNA/DNA/protein
Technical data EU / UK : 230V AC 125W 10,000 – 33,000rpm; US:120V ~ 1.15A 60Hz 5,000 – 35,000rpm
Technology Handheld rotor-stator homogenizer
Throughput 1 sample per run
Typical run time 15–120 seconds

Kit Handbooks
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For low-throughput disruption of biological samples using disposable probes
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ディスポーザブルプローブを用いる、低スループットの生体サンプルを破砕
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