- Specific and sensitive detection of even low-copy targets
- Optimized master mix for reliable results without optimization
- 5-minute enzyme activation and fast cycling conditions
- Accurate detection of a wide range of template amounts
- Universal protocol for all standard and fast cyclers
The QuantiFast SYBR Green PCR Kit delivers fast and specific quantification of gDNA or cDNA targets by real-time PCR or two-step RT-PCR using SYBR Green I detection. Q-bond technology and an optimized, ready-to-use master mix enable shorter real-time PCR run times, not only on fast cyclers with short ramping times, but also on standard cyclers. The combination of a hot start and a unique PCR buffer system in the ready-to-use master mix ensures highly sensitive qPCR on any real-time cycler without the need for optimization. For convenience, the master mix can be stored at 2–8°C.