For qualitative or semi-quantitative detection of the JAK2 V617F/G1849T mutation using real-time PCR
  • Reliable qualitative or semi-quantitative detection
  • Multiplex analysis with no need for optimization
  • Easy workflow with ready-to-use solutions
 
The ipsogen JAK2 MutaScreen Kits are intended for research use only and are not for use in diagnostic procedures. The kits are for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. The kits provide reagents optimized for reliable and sensitive detection, and qualitative or semi-quantitative analysis of the JAK2 V617F/G1849T mutation in genomic DNA.
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ipsogen JAK2 MutaScreen Kit (10)
For 10 reactions: V617F Positive Control, V617F Negative Control, V617F Cut-Off Sample, Primers and Probes Mix JAK2 wild-type and JAK2 V617F
673012
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ipsogen JAK2 MutaScreen Kit (24)
For 24 reactions: V617F Positive Control, V617F Negative Control, V617F Cut-Off Sample, Primers and Probes Mix JAK2 wild-type and JAK2 V617F
673013
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ipsogen JAK2 MutaScreen RS Kit (19)
For 19 reactions: V617F Positive Control, V617F Negative Control, V617F Reference Scale, Primers and Probes Mix JAK2 wild-type and JAK2 V617F
673113
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For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
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JAK2 mutation site identification.|Representative allelic discrimination experiments for qualitative detection of the JAK2 V617F/G1849T mutation in genomic DNA.|Representative allelic discrimination experiments for semi-quantitative detection of the JAK2 V617F/G1849T mutation in genomic DNA.|
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|M1–M6 represent reference scale samples.|
Performance
To ensure highest accuracy, ipsogen JAK2 MutaScreen Kits have been optimized to detect the JAK2 V617F/G1849T mutation and use DNA positive and negative controls for qualitative analysis (see figures Representative allelic discrimination experiments for qualitative detection of the JAK2 V617F/G1849T mutation in genomic DNA and Representative allelic discrimination experiments for semi-quantitative detection of the JAK2 V617F/G1849T mutation in genomic DNA). ipsogen JAK2 MutaScreen Kits provide a DNA 2% cut-off sample (COS) positivity and a minimum of 25 ng of input sample DNA is recommended.
Principle
ipsogen JAK2 MutaScreen Kits are ready-to-use kits for the detection, qualitative, or semi-quantitative analysis of the JAK2 V617F/G1849T somatic mutation due to a single nucleotide polymorphism (SNP) in genomic DNA (see figure JAK2 mutation site identification). The kits provide reliable and reproducible results. ipsogen JAK2 MutaScreen Kits provide a DNA cut-off sample (COS), a DNA positive control (PC), and a negative control (NC) for JAK2 V617F. Use of the cut-off sample and controls enables accurate qualitative analysis of genotyping results. The ipsogen JAK2 MutaScreen RS Kit provides a V617F reference scale.
Procedure
The first step is extraction and determination of quality and quantity of genomic DNA. The second step is the amplification of DNA by real-time PCR. Using the ipsogen JAK2 MutaScreen Kits provide reliable detection, qualitative, or semi-quantitative analysis of the JAK2 V617F/G1849T mutation. Simply start the reaction using the optimized protocols described in the kit handbook.
Applications
ipsogen JAK2 MutaScreen Kits enable sensitive and reliable detection, qualitative, or semi-quantitative analysis of the JAK2 V617F/G1849T mutation for research use only.