mericon Pathogen Detection Assays

For real-time PCR detection of pathogen DNA in food or animal feed

  • Highly sensitive and specific detection of a broad range of pathogens
  • Single, streamlined detection protocol for all targets
  • Dedicated mericon bacterial DNA isolation kits available
  • PCR run time of 73 minutes on the QIAGEN Rotor-Gene Q
  • Full license for PCR without additional costs

mericon Pathogen Detection Assays are a suite of kits for the target-specific detection of bacterial pathogen DNA in food, animal feed, and pharmaceutical products. All of the assays use the same real-time PCR-based protocol. They are part of the comprehensive QIAGEN food testing portfolio, which also features dedicated sample preparation kits and assays for ingredient authentication and GMO DNA detection. mericon assays perform optimally on the Rotor-Gene Q, but have also been validated for block thermal cyclers.

Quantity Product Cat. no. Price
 
 
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Quantity Product Cat. no. Price Sum
 
mericon Campylobacter spp Kit (24)
For 24 reactions: PCR Assay Campylobacter spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290033
Inquire
 
mericon Campylobacter spp Kit (96)
For 96 reactions: PCR Assay Campylobacter spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290035
Inquire
 
mericon Campylobacter triple Kit (24)
For 24 reactions: PCR Assay Campylobacter triple, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290043
Inquire
 
mericon Campylobacter triple Kit (96)
For 96 reactions: PCR Assay Campylobacter triple, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290045
Inquire
 
mericon Cronobacter spp Kit (24)
For 24 reactions: PCR Assay Cronobacter spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290063
Inquire
 
mericon Cronobacter spp Kit (96)
For 96 reactions: PCR Assay Cronobacter spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290065
Inquire
 
mericon Listeria spp Kit (24)
For 24 reactions: PCR Assay Listeria spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290123
Inquire
 
mericon Listeria spp Kit (96)
For 96 reactions: PCR Assay Listeria spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290125
Inquire
 
mericon L. monocytogenes Kit (24)
For 24 reactions: PCR Assay L. monocytogenes, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290023
Inquire
 
mericon L. monocytogenes Kit (96)
For 96 reactions: PCR Assay L. monocytogenes, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290025
Inquire
 
mericon S. aureus Kit (24)
For 24 reactions: PCR Assay S. aureus, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290073
Inquire
 
mericon S. aureus Kit (96)
For 96 reactions: PCR Assay S. aureus, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290075
Inquire
 
mericon Salmonella spp Kit (24)
For 24 reactions: PCR Assay Salmonella spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290013
Inquire
 
mericon Salmonella spp Kit (96)
For 96 reactions: PCR Assay Salmonella spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290015
Inquire
 
mericon Shigella spp Kit (24)
For 24 reactions: PCR Assay Shigella spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290103
Inquire
 
mericon Shigella spp Kit (96)
For 96 reactions: PCR Assay Shigella spp, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290105
Inquire
 
mericon VTEC stx 1/2 Kit (24)
For 24 reactions: PCR Assay VTEC stx 1/2, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290053
Inquire
 
mericon VTEC stx 1/2 Kit (96)
For 96 reactions: PCR Assay VTEC stx 1/2, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290055
Inquire
 
mericon Y. enterocolitica Kit (24)
For 24 reactions: PCR Assay Y. enterocolitica, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290113
Inquire
 
mericon Y. enterocolitica Kit (96)
For 96 reactions: PCR Assay Y. enterocolitica, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution
290115
Inquire
 
mericon Quant Legionella spp Kit

For 96 reactions: PCR Assay Quant Legionella spp, Internal Control, Standard DNA, Quant Control DNA, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution

290085
$1,044.00
$0.00
 
mericon Quant L. pneumophila Kit (96)

For 96 reactions: PCR Assay Quant L. pneumophila, Internal Control, Standard DNA, Quant Control DNA, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water, 50x ROX Dye Solution

290095
$1,044.00
$0.00
mericon Pathogen Detection Assays are intended for molecular biology applications in food, animal feed, and pharmaceutical product testing. These products are not intended for the diagnosis, prevention, or treatment of a disease.
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Performance
mericon Campylobacter spp Kit

The mericon Campylobacter spp Kit is for the specific detection of several Campylobacter subspecies in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of Campylobacter DNA in a reaction and it exhibits high specificity for pathogens this genus. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Campylobacter spp Kit cross-reactivity study").

mericon Campylobacter triple Kit

The mericon Campylobacter triple Kit is designed for the detection of Campylobacter jejuni, C. coli, and C. laridis in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of C. jejuni, C. coli, or C. laridis DNA in a reaction and it exhibits high specificity. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Campylobacter triple Kit cross-reactivity study").

mericon Cronobacter spp Kit

The mericon Cronobacter spp Kit is for the specific detection of Chronobacter subspecies in enrichment cultures of food and animal feed samples. Due to a recent change in taxonomy and according to regulation of the EU Commission (365/2010) the name Cronobacter spp replaces Enterobacter sakazakii. The assay can detect as few as 10 copies of Cronobacter DNA in a reaction and it exhibits high specificity. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Cronobacter spp Kit cross-reactivity study").

mericon Quant Legionella spp Kit

The mericon Quant Legionella spp Kit is for the specific detection and quantification of several Legionella species in water after concentration (e.g., by filtration). The assay can detect as few as 10 copies of Legionella DNA in a reaction and it exhibits high specificity for pathogens of that genus. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Quant Legionella spp Kit cross-reactivity study"). 

mericon Quant L. pneumophila Kit

The mericon Quant L. pneumophila Kit is for the specific detection and quantification of Legionella pneumophila in water after concentration (e.g., by filtration). The assay can detect as few as 10 copies of L. pneumophila DNA in a reaction and it exhibits high specificity for L. pneumophila . In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Quant L. pneumophila Kit cross-reactivity study").

mericon Listeria spp Kit

The mericon Listeria spp Kit is for the specific detection of several Listeria subspecies in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of Listeria DNA in a reaction and it exhibits high specificity for pathogens of this genus. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Listeria Kit cross-reactivity study").

mericon L. monocytogenes Kit

The mericon L. monocytogenes Kit is for the specific detection of Listeria monocytogenes in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of L. monocytogenes DNA in a reaction and it exhibits high specificity. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens, even closely-related Listeria species (see the table "Results of mericon L. monocytogenes Kit cross-reactivity study").

mericon Salmonella spp Kit

The mericon Salmonella spp Kit is for the specific detection of several Salmonella subspecies in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of Salmonella DNA in a reaction and it exhibits high specificity for pathogens of this genus. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Salmonella spp Kit cross-reactivity study").

mericon Shigella spp Kit

The mericon Shigella spp Kit is for the specific detection of several Shigella subspecies in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of Shigella DNA in a reaction and it exhibits high specificity for pathogens of this genus. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Shigella spp Kit cross-reactivity study").

mericon S. aureus Kit

The mericon S. aureus Kit is for the specific detection of Staphylococcus aureus in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of S. aureus DNA in a reaction and it exhibits high specificity. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon S. aureus Kit cross-reactivity study").

mericon VTEC stx1/2 Kit

The mericon VTEC stx1/2 Kit is for the specific detection of the strains of Escherichia coli that produce verotoxins, also called Shiga toxins, in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of verotoxin-producing E. coli DNA in a reaction and it exhibits high specificity. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon VTEC stx1/2 Kit cross-reactivity study").

mericon Y. enterocolitica Kit

The mericon Y. enterocolitica Kit is designed for the detection of Yersinia enterocolitica in enrichment cultures of food and animal feed samples. The kit specifically detects human pathogen serotypes 0:3, 0:5, 0:6, 0:8, 0:9, and 0:5:27. The assay can detect as few as 10 copies of Yersinia enterocolitica DNA in a reaction and exhibits high specificity. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Y. enterocolitica Kit cross-reactivity study").

Results of mericon Campylobacter spp Kit cross-reactivity study
Pathogen Result Pathogen Result
Campylobacter jejuni + Campylobacter coli +
Campylobacter laridis + Campylobacter upsaliensis +
Salmonella spp. VTEC stx1
Clostridium perfringens VTEC stx2
Legionella erythra Yersinia enterocolitica
Staphylococcus aureus Listeria monocytogenes
Results of mericon Campylobacter triple Kit cross-reactivity study
Pathogen Result Pathogen Result
Campylobacter jejuni + Campylobacter coli +
Campylobacter laridis + Campylobacter upsaliensis
Salmonella spp. VTEC stx1
Clostridium perfringens VTEC stx2
Legionella pneumophila Yersinia enterocolitica
Staphylococcus aureus Listeria monocytogenes
Escherichia coli Bacillus cereus
Results of mericon Cronobacter spp Kit cross-reactivity study
Pathogen Result Pathogen Result
Cronobacter sakazakii + Listeria monocytogenes
Staphylococcus aureus Escherichia coli
Salmonella spp. Legionella erythra
Clostridium perfringens Bacillus cereus
Legionella pneumophila Yersinia enterocolitica
Shigella flexneri VTEC stx1
Campylobacter jejuni VTEC stx2
Results of mericon Legionella spp Kit cross-reactivity study
Pathogen Result Pathogen Result
Legionella pneumophila + Legionella erythra +
Salmonella thyphimurium Escherichia coli
Shigella flexneri Bacillus cereus
Clostridium perfringens Yersinia enterocolitica
Campylobacter jejuni
Results of mericon L. pneumophila Kit cross-reactivity study
Pathogen Result Pathogen Result
Legionella pneumophila + Legionella erythra
Salmonella thyphimurium Escherichia coli
Shigella flexneri Bacillus cereus
Clostridium perfringens Yersinia enterocolitica
Campylobacter jejuni
Results of mericon Listeria spp Kit cross-reactivity study
Pathogen Result Pathogen Result
Listeria monocytogenes + Cronobacter sakazakii
Listeria welshimeri + Legionella pneumophila
Listeria seeligeri + Staphylococcus aureus
Listeria ivanovii + Bacillus cereus
Listeria innocua + Yersinia enterocolitica
Listeria marthii + VTEC
Listeria grayi + Legionella erythra
Listeria rocoutiae + Shigella flexneri
Campylobacter jejuni Clostridium perfringens
Escherichia coli
Results of mericon L. monocytogenes Kit cross-reactivity study
Pathogen Result Pathogen Result
Listeria monocytogenes + Cronobacter sakazakii
Clostridium perfringens Bacillus cereus
Staphylococcus aureus Legionella erythra
Salmonella spp. Campylobacter jejuni
Yersinia enterocolitica Legionella pneumophila
Shigella flexneri VTEC stx1
Escherichia coli VTEC stx2
Listeria innocua Listeria welshimeri
Listeria ivanovii spp. Ivanovii Listeria grayi
Listeria seeligeri
Results of mericon Salmonella spp Kit cross-reactivity study
Pathogen Result Pathogen Result
Salmonella spp + Cronobacter sakazakii
Clostridium perfringens Bacillus cereus
Staphylococcus aureus Legionella erythra
Listeria monocytogenes Campylobacter jejuni
Yersinia enterocolitica Legionella pneumophila
Shigella flexneri VTEC stx1
Escherichia coli VTEC stx2
Results of mericon Shigella spp Kit cross-reactivity study
Pathogen Result Pathogen Result
Shigella spp. + Listeria monocytogenes
Staphylococcus aureus Escherichia coli
Salmonella spp. Bacillus cereus
Clostridium perfringens Yersinia enterocolitica
Campylobacter jejuni Legionella erythra
VTEC stx1 VTEC stx2
Results of mericon S. aureus Kit cross-reactivity study
Pathogen Result Pathogen Result
Staphylococcus aureus + Listeria monocytogenes
Cronobacter sakazakii Escherichia coli
Salmonella spp. Legionella erythra
Clostridium perfringens Bacillus cereus
Legionella pneumophila Yersinia enterocolitica
Shigella flexneri VTEC stx1
Campylobacter jejuni VTEC stx2
Results of mericon VTEC stx1/2 Kit cross-reactivity study
Pathogen Result Pathogen Result
VTEC stx1 + VTEC stx2 +
Campylobacter jejuni Escherichia coli
Salmonella spp. Legionella erythra
Clostridium perfringens Bacillus cereus
Legionella pneumophila Yersinia enterocolitica
Staphylococcus aureus Listeria monocytogenes
Results of mericon Y. enterocolitica Kit cross-reactivity study
Pathogen Result Pathogen Result
Yersinia enterocolitica + Listeria monocytogenes
non-pathogenic Yersinia
enterocolitica
Escherichia coli
Staphylococcus aureus Bacillus cereus
Clostridium perfringens Salmonella spp.
Campylobacter jejuni Legionella erythra
VTEC stx1 VTEC stx2
The mericon Quant L. pneumophila Kit is for the specific detection and quantification of Legionella pneumophila in water after concentration (e.g., by filtration). The assay can detect as few as 10 copies of L. pneumophila DNA in a reaction and it exhibits high specificity for L. pneumophila . In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogensThe mericon Quant Legionella spp Kit is for the specific detection and quantification of several Legionella species in water after concentration (e.g., by filtration). The assay can detect as few as 10 copies of Legionella DNA in a reaction and it exhibits high specificity for pathogens of that genus. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table "Results of mericon Quant Legionella spp Kit cross-reactivity study").
Principle

Detection of pathogens using real-time PCR is based on the amplification of a specific region of the relevant pathogen genome. The amplified product is detected using target-specific fluorescent probes that bind to it. As the PCR product accumulates, there is an increased fluorescent signal from the bound probes. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the detection of the accumulating PCR product without having to re-open the reaction tubes afterward.

mericon PCR Assays include an internal control to control potential inhibiton. This aids in the straightforward interpretation of the results (see the table "Summary of possible PCR outcomes").

The PCR primer set for each assay is highly specific and targets a unique and conserved region of the tested pathogen genome that has been bioinformatically and experimentally verified.

Summary of possible PCR outcomes
Internal control amplifiedTested DNA amplified Meaning
+ + Sample is positive for the target DNA
+ Sample is negative for the target DNA
PCR failed due to inhibition
Procedure

DNA is extracted from an enrichment culture of a sample of food or animal feed using a dedicated sample preparation kit. The mericon Bacteria Kit is for the isolation of bacterial DNA from Gram-negative bacteria. The mericon Bacteria Plus Kit is for the isolation of bacterial DNA from Gram-positive bacteria.

The isolated, pure bacterial DNA is assayed using a streamlined real-time PCR protocol that returns results in 73 minutes on QIAGEN's Rotor-Gene Q. The protocol has also been validated for use on other thermal block cyclers.

The results are displayed as easy-to-interpret PCR curves.

Applications

mericon Pathogen Detection Assays are for real-time PCR-based detection of specific bacterial pathogens in food, animal feed, and pharmaceutical products.

Feature
Specifications
Quantitative/qualitative Qualitative (Quantitative for Quant L pneumophilia and Quant Legionella spp)
Recommended sample prep mericon DNA Bacteria Kit (Gram-negative bacteria) or mericon DNA Bacteria Plus Kit (Gram-positive bacteria) or QIAamp UCP Pathogen Mini Kit (Legionella-specific)
RUO/CE/ASR RUO
Sample type Bacterial preculture from food and water samples after concentration, e.g. filtration (for Legionella- specific Kits)
Thermal cycler Rotor-Gene Q and other cyclers
What detected DNA of respective pathogen