How do I prepare Buffer MP?

Here is the protocol for preparing buffer MP:

 

  1. Dissolve 3.3 g citric acid monohydrate in 3 ml high-purity water at room temperature (21°C) in a 10 ml tube.
  2. Stir the solution at 200 rpm for 5 min.
  3. Filter the solution through a 0.2 μm sterile filter using a syringe to give a final volume of 6 ml Buffer MP.
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