Can I use a custom master mix instead of a QIAGEN master mix?

The QIAcuity Digital PCR System is designed to deliver precise and multiplexed quantification results for mutation detection, copy number variation (CNV), gene expression studies, gene-editing analysis, and many more. This nanoplate-based system seamlessly integrates a standard dPCR workflow of partitioning, thermocycling and imaging into a walk-away automated platform with minimal hands-on time. The system is used in conjunction with the QIAcuity nanoplates, reagents and assays. Explore the virtual demo to learn more about QIAcuity.

The QIAcuity Nanoplates are microfluidic digital PCR plates that enable running 8, 24 or 96 samples with up to 8500 or 26,000 partitions per well. All four nanoplates are designed to run on the QIAcuity digital PCR instruments. The nanoplates can only be used with the QIAcuity Digital PCR System. Use the dedicated QIAcuity Nanoplate Adapter when performing automated liquid handling and PCR setup in a QIAcuity Nanoplate using the QIAgility. Once done, load the plate onto the QIAcuity Digital PCR System for the dPCR reaction. Would you like to learn more about the product and be contacted by one of our dPCR specialists? Sign in here, and we will get in touch with you shortly.

The QIAcuity Probe PCR Kit contains a 4x concentrated, ready to use Master Mix optimized for microfluidic use in the QIAcuity Nanoplates. The kit enhances the specificity and efficiency of probe-based digital PCR to provide accurate, singleplex, or up to 5-plex analysis. The QIAcuity Probe Master Mix is optimized to increase specificity for accurate quantification of gDNA or cDNA on the QIAcuity dPCR instruments. The kit works in conjunction with the QIAcuity Digital PCR System and the QIAcuity Nanoplates. Would you like to learn more about the product and be contacted by one of our dPCR specialists? Sign in here, and we will get in touch with you shortly.

The QIAcuity EG PCR Kit contains a 3x concentrated, ready-to-use Master Mix optimized for microfluidic use in the QIAcuity Nanoplates. The kit improves the specificity and efficiency of dye-based digital PCR to provide accurate quantitation analysis. An intercalating dye EvaGreen binds to double-stranded DNA and enhances the quantitative accuracy of gDNA or cDNA measurements on the QIAcuity dPCR instruments. The kit works in conjunction with the QIAcuity Digital PCR System and the QIAcuity Nanoplates. Would you like to learn more about the product and be contacted by one of our dPCR specialists? Sign in here, and we will get in touch with you shortly.