What causes wide peaks?

Sodium hydroxide carry-over, too much template, and incorrectly stored reagent are the most common causes of wide peaks. Ensure correct volume of denaturation buffer is used on the vacuum prep station. Use 5–20 µl of PCR product depending on instrument models. Store reagent as described in the handbook.

 

If the issue persists, please send the run files (see Instruction for run file export) to QIAGEN Technical Service for further assistance.