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Isolation of BAC DNA using the QIAGEN® Plasmid Midi Kit - (EN)
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This procedure has been used successfully for isolation of 150-250 kb BAC DNA from a mouse-BAC library cloned in pBeloBAC11 from
Escherichia coli
strain HB101/r. The yield of BAC DNA from 100 ml culture was typically 20-40 μg.
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