Do you have a protocol for disrupting animal tissues with TissueLyser for the purpose of extracting proteins?

We have not specifically tested this application. However, several customers have successfully used QIAGEN’s TissueLyser II for disruption of animal tissues to extract proteins. We suggest:

1. Pre-cool one 5 mm stainless steel bead and the TissueLyser II adapter at 4°C.
2. Chill the appropriate lysis buffer by incubating at 4°C prior to use
3. Freeze tissue in dry ice and immediately add 1 cold bead plus 200–300 µL of chilled lysis buffer to the TissueLyser II adapter
4. Disrupt twice, each time for 30 s at 20 Hz

Please be advised that these are suggestions only and have not been thoroughly tested by QIAGEN. It is the user’s responsibility to test and further optimize if necessary.