✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Cat. No. / ID: 74171
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Cell line | Source | RNA yield (µg per 105 cells)* |
---|---|---|
HeLa | Human cervical carcinoma | 1.6 |
LMH | Chicken hepatoma | 1.3 |
COS-7 | Monkey kidney, SV-40 transformed | 3.1 |
Huh7 | Human hepatoma | 2.0 |
Jurkat | Human T-cell leukemia | 1.4 |
K-562 | Human chronic myelogenous leukemia in blast crisis | 1.9 |
The RNeasy 96 QIAcube HT Kit enables automated purification of total RNA or total RNA, including miRNA, from 5 x 105 animal or human cells or <40 mg tissue using the QIAcube HT instrument. Six instrument protocols are available, depending on the starting material and desired size distribution of purified RNA. Cell samples can be purified directly using the RNeasy 96 QIAcube HT Kit and the dedicated kit protocol. Tissue samples (<40 mg) and samples for purification of small RNA are processed following sample lysis in QIAzol Lysis Reagent. The procedures yield high-quality RNA that performs well in downstream applications.
The RNeasy 96 QIAcube HT Kit combines the selective binding properties of a silica-based membrane with a high-throughput 96-well format, and is designed for fully-automated, simultaneous processing of 24–96 samples on the QIAcube HT instrument.
Specification | Description |
---|---|
Number of samples | 24–96 samples (to be processed in increments of 8) |
Sample input volume | Up to 5 x 105 cells, homogenized in 140 μl Buffer RLT |
Elution volume | 110 μl |
Duration | 96 samples in approximately 93 or 114* minutes 24 samples in approximately 54 or 73* minutes |
Specification | Description |
---|---|
Number of samples | 24–96 samples (to be processed in increments of 8) |
Sample input volume | 350 μl aqueous phase from up to 40 mg frozen or 20 mg stabilized tissue homogenized in 750 μl QIAzol Lysis Reagent |
Elution volume | 110 μl |
Duration | 96 samples in approximately 73 or 93* minutes 24 samples in approximately 34 or 54* minutes |
Specification | Description |
---|---|
Number of samples | 24–96 samples (to be processed in increments of 8) |
Sample input volume | 350 μl aqueous phase from up to 40 mg frozen or 20 mg stabilized tissue homogenized in 750 μl QIAzol Lysis Reagent |
Elution volume | 110 μl |
Duration | 96 samples in approximately 78 minutes 24 samples in approximately 38 minutes |
The RNeasy 96 QIAcube HT Kit provides efficient, high-throughput RNA sample preparation for research use in fields such as drug screening and basic research. The purified RNA is ready to use in any downstream application, including: