Qproteome Albumin/IgG Depletion Kit

For depletion of albumin and IgG from human serum and plasma samples

Efficient removal of human albumin and IgG
Highly specific depletion using immobilized monoclonal antibodies
Fast and easy-to-use spin column format

The kit contains spin columns for depletion of albumin and IgG from human serum or plasma samples. Spin Column Adapter Rings are required for automation of this kit on the QIAcube.

Quantity	Product	Cat. no.	Price	Sum
	Qproteome Albumin/IgG Depletion Kit For albumin/IgG depletion of 6 serum or plasma samples: Albumin/IgG Depletion Spin Columns (6)	37521	$334.00	$0.00

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The Qproteome Albumin/IgG Depletion Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

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Qproteome Albumin/IgG Depletion Kit procedure.|Albumin/IgG depletion facilitates analysis of low-abundance proteins.|

|Coomassie stained 2D-PAGE gels showing [A] non-depleted and [B] depleted (right) plasma samples.|

Performance

The Qproteome Albumin/IgG Depletion Kit provides efficient and specific removal of highly abundant albumin and IgG from human serum and plasma samples (see figure “Albumin/IgG depletion facilitates analysis of low-abundance proteins.")

Principle

Albumin and IgG in the sample are bound by antibodies immobilized on a solid support in the spin columns.

Procedure

Diluted samples are applied to the resin in the spin columns, which are then sealed and incubated on an end-over-end shaker. Plasma and serum proteins, depleted of albumin and IgG, are recovered by centrifugation (see "Qproteome Albumin/IgG Depletion Kit procedure").

Applications

The Qproteome Albumin/IgG Depletion Kit facilitates analysis of less abundant proteins.

Feature	Specifications
Applications	SDS-PAGE, mass spectrometry
Binding capacity/yield	0.5–0.8 mg
Fractions isolated	One fraction
Sample size	<25 µl
Species	Human
Start material	Serum, plasma

Kit Handbooks

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	(EN) - Albumin and IgG Depletion Handbook	Format pdf File size 218KB	Download
	Qproteome Albumin/IgG Depletion プロトコールとトラブルシューティング - (JA) ヒト血漿あるいは血清サンプルからのアルブミンおよびIgGの分離; マウス・ラット血漿あるいは血清サンプルからのアルブミンの分離	Format pdf File size 51KB	Download

FAQs

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	1034 - What is the resin in the Qproteome Murine Albumin and Qproteome Albumin/IgG Depletion Kits?	Format FAQ File size	View
	1090 - What is the protein loading capacity per column of the Qproteome Albumin/IgG Depletion Kit?	Format FAQ File size	View
	1173 - Can the Spin Columns of the Qproteome Albumin/IgG Depletion Kit be reused?	Format FAQ File size	View
	1035 - How do I perform an Acetone Precipitation for concentrating and desalting protein samples?	Format FAQ File size	View
	807 - Will acetone precipitation recommended in the Qproteome Protocols denature Protein?	Format FAQ File size	View
	753 - Where can I find protocols for the processing of samples not mentioned in the QIAGEN handbooks?	Format FAQ File size	View

Scientific Posters

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	(EN) - Medium- and high-throughput depletion using the QIAcube instrument and a 96-well format	Format pdf File size 384KB	Download
	(EN) - A range of ready-to-use kits reduces complexity of protein samples for proteomics studies	Format pdf File size 1101KB	Download

Technical information

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	(EN) - Standardized and reproducible protein fractionation and depletion with Qproteome Kits	Format pdf File size 849KB	Download