For rapid, high-precision automated PCR setup
- Automated PCR setup in all formats
- Convenient, easy-to-use software
- Standardized results and increased productivity
- Elimination of manual pipetting steps
- Seamless integration with QIAGEN sample technologies
The QIAgility is a compact benchtop instrument that enables rapid, high-precision PCR setup. The unmatched versatility of the QIAgility means that almost all tube and plate formats are supported, as well as Rotor-Discs for the Rotor-Gene Q. The QIAgility is operated via a computer with easy-to-use software. For PCR success at first attempt, use verified Q Protocols for PCR setup in combination with QIAGEN PCR kits and enzymes for end-point and real-time PCR.
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QIAgility HEPA/UV (incl. PC)
Robotic workstation for automated PCR setup (with UV light and HEPA filter), notebook computer, and QIAgility Software: includes 1 year warranty on parts and labor
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9001611
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QIAgility System HEPA/UV (incl. PC)
Robotic workstation for automated PCR setup (with UV light and HEPA filter), notebook computer, and QIAgility Software: includes installation and training, 1-year warranty on parts and labor
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9001532
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QIAgility Priority Package HEPA/UV (incl. PC)
Robotic workstation for automated PCR setup (with UV light and HEPA filter), notebook computer, and QIAgility Software: includes Priority Package with software, installation, training, 2-year warranty on parts and labor, and 2 preventive maintenance visits
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9001903
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QIAgility Priority Package Plus HEPA/UV (incl. PC)
Robotic workstation for automated PCR setup (with UV light and HEPA filter), notebook computer, and QIAgility Software: includes Priority Package with software, installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits
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9001904
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QIAgility HEPA/UV
Robotic workstation for automated PCR setup (with UV light and HEPA filter), and QIAgility Software: includes 1 year warranty on parts and labor
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9001731
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QIAgility System HEPA/UV
Robotic workstation for automated PCR setup (with UV light and HEPA filter), and QIAgility Software: includes installation and training, 1-year warranty on parts and labor
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9001908
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The QIAgility, if used in combination with QIAGEN kits indicated for use with the QIAgility instrument, is intended for the applications described in the respective QIAGEN kit handbooks. If the QIAgility instrument is used with kits other than QIAGEN kits, it is the user’s responsibility to validate the performance of such product combination for any particular application.
Eliminate manual pipetting steps.|Exceptional reproducibility and accurate series dilutions.|Accurate automated reaction setup.|Instrument worktable.|Easy-to-use software.|
Prp, HugL, and Cyst were amplified from human genomic DNA (10 ng). PCR reactions were set up using the TopTaq Master Mix Kit, in replicates of 2, using the QIAgility and manually. After cycling, reactions were analyzed by capillary electrophoresis, using the QIAxcel.|A 2-fold series dilution of human genomic DNA (30 mg to 0.6 mg) was performed using the QIAgility. IL1R2 was amplified from these dilutions, using the Rotor-Gene Q and the QuantiFast Probe PCR Kit. Reactions were set up in replicates of 5. DNA yields were quantified by quantitative, real-time PCR, where the Rotor-Gene Q was also used for analysis. Plots show [ A] highly precise and reproducible replicates with a 1 cycle difference between each 2-fold dilution and [ B] the standard curve.
|IL12RB1 was amplified from leukocyte RNA (1, 10, and 100 ng), using the Rotor-Gene Q and the QuantiFast Probe RT-PCR Kit. Replicates of 3 were set up manually and using the QIAgility. DNA yields were quantified by quantitative, real-time RT-PCR, where the Rotor-Gene Q was also used for analysis.|Supports Master Mix Block, Reagent Block, tips, and plates/tubes.|
The worktable setup is mirrored in the main software window.
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Performance
Automated PCR setup is rapid and reliable and eliminates manual pipetting steps that can be prone to human error (see figures "Accurate automated reaction setup", "Eliminate manual pipetting steps", and Exceptional reproducibility and accurate series dilutions).
The advanced design of the QIAgility delivers improved process safety, giving you peace of mind. An in-built sensor ensures that the instrument pauses when the hood is opened during a run, protecting users from moving parts. An optional UV light ensures effective worktable decontamination and an optional HEPA filter provides positive clean air pressure throughout PCR setup. These safety features are complemented by intelligent safety warnings from the software, should an error occur.
In addition to PCR setup, the flexibility of the QIAgility allows the user to program an unrivaled range of high-precision pipetting applications (see table Speed and versatility), which includes:
- Normalization of DNA and RNA concentration
- Transfer of liquid samples from one tube format to another
- Serial dilutions with variable dilution ratios
- Restriction digest setup
- Sample pooling
- Selective pipetting from archived sample banks
| Normalization |
One-step dilution |
96 |
No |
108 x T50* |
28 |
| Reformatting |
1:1 transfer |
96 |
No |
96 x T50 |
17 |
| Plate replication |
1:4 transfer |
96–384 |
No |
154 x T50 |
71 |
| Plate replication |
1:4 transfer |
96–384 |
Yes |
96 x T50 |
46 |
| Serial dilution |
16 concentrations |
16 dilutions |
No |
32 x T50 |
7 |
| Repetitive aliquoting |
Primer solution (2 ml) into aliquots (20 µl) |
96 aliquots |
Yes (8 times) |
12 x T50 |
10 |
| Repetitive aliquoting |
Primer solution (2 ml) into aliquots (20 µl) |
96 aliquots |
No |
96 x T50 |
17 |
| Preparation of standards |
6 concentrations |
6 dilutions |
No |
11 x T50
5 x T200† |
3 |
| Master mix preparations |
8 components for 96 samples |
96 |
Yes (8 times) |
10 x T 200 |
6 |
| PCR setup |
Premixed master mix |
96 |
Yes (8 times) |
108 x T50 |
36 |
| PCR setup |
Mastermix preparation |
96 |
Yes (8 times) |
110 x T50
10 x T200 |
39 |
| PCR setup |
Premixed master mix and prediluted |
96 |
Yes (8 times) |
108 x T50
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36 |
| PCR setup |
Master mix preparation and standard dilution |
96 |
Yes (8 times) |
115 x T 50
15 X T 200 |
41 |
Principle
The QIAgility uses a single-channel pipet to automate PCR setup — up to 96 PCR reactions can be set up in approximately 30 minutes. Liquid-level sensing, facilitated by conductive filtered tips, enables high-precision, standardized pipetting (see table Outstanding precision). Automated PCR setup is rapid and reliable and eliminates manual pipetting steps that can be prone to human error (see figures "Accurate automated reaction setup" and "Eliminate manual pipetting steps"). The QIAgility fully automates trusted QIAGEN PCR and RT-PCR, and real-time PCR and RT-PCR kits, and can set up multiple master mixes and can process multiple PCR setups in parallel.
| 1 |
0.99 |
0.06 |
5.64 |
| 2 |
2.03 |
0.05 |
2.20 |
| 5 |
5.02 |
0.03 |
0.56 |
| 10 |
9.99 |
0.02 |
0.24 |
| 20 |
19.97 |
0.04 |
0.22 |
| 50 |
49.99 |
0.05 |
0.10 |
| 100 |
100.84 |
0.14 |
0.14 |
| 150 |
150.79 |
0.25 |
0.17 |
| 200 |
198.82 |
0.26 |
0.13 |
The advanced design of the QIAgility delivers improved process safety, giving you peace of mind. An in-built sensor ensures that the instrument pauses when the hood is opened during a run, protecting users from moving parts. An optional UV light ensures effective worktable decontamination and an optional HEPA filter provides positive clean air pressure throughout PCR setup. These safety features are complemented by intelligent safety warnings from the software, should an error occur.
Procedure
The instrument worktable is set up with a Master Mix Block, a Reagent Block, tips and plates/tubes (see figure " Instrument worktable"). The QIAgility software simulates the instrument worktable, making it exceptionally easy to use by both automation novices and more experienced laboratory personnel. Simply select a program, set up the worktable, and walk away during setup. Samples are ready for immediate use in downstream applications.
Applications
The QIAgility is highly suited for laboratories performing PCR and RT-PCR in applications such as:
- Gene expression analysis
- Virus detection
- Genotyping
- Cloning
- Pathogen detection
Software
QIAgility Software simulates the instrument worktable, making it exceptionally easy to use (see figure " Easy-to-use software"). Fast setup and development of run files are facilitated by wizards, which guide the user through the software procedures in a stepwise fashion. The user can also download Q Protocols and modify them according to their needs. Q Protocols are run files that were developed by QIAGEN to directly facilitate specific PCR applications. The software highlights the position of the pipetting head on the screen as it moves, allowing the progress of a run to be followed in real-time.
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Feature
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Specifications
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Accessories (supplied)
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2 x Adapters (96 x 0.2 ml PCR tubes); 1 x Flip-Cap Adapter (32 x 1.5 ml tapered tubes); 1 x Flip-Cap Tube Lifter (32 x 1.5 ml tapered tubes); 1 x Reagent Block (8 x 1.5 ml tapered tubes, 8 x 2 ml free-standing tubes, and 16 x 200 µl PCR tubes); 1 x Master Mix Block (1 x 5 ml and 4 x 1.5 ml tubes); 1 x Master Mix Block (5 x 5 ml tubes); 4 x Tip Rack Holders
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Communications
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USB and RS232
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Conductive Filtered Tips
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50 µl and 200 µl Conductive Filtered Tips for liquid-level sensing
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Consumables (supplied)
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10 racks of 96 x 50 µl Conductive Filtered Tips; 50 x 5 ml free-standing tubes; 5 x Tip Receptacle Boxes
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Dimensions
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Height: 45 cm (17.7 in.), hood closed; Width: 55 cm (21.6 in.); Depth: 63 cm (24.8 in.), without cables
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Electrical
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100–240 V, 250 VA, 50–60 Hz, 5 A fuse
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HEPA filter (optional)
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Dual-cartridge 3M Air-Mate™ HEPA (High Efficiency Particulate Air) filter system provides positive pressure clean air within the closed hood. Available only with initial purchase, in combination with the UV lamp. Replacement filters can be purchased separately.
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Humidity
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Operating humidity: 40–70%
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Liquid-level sensing
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Minimum detection volume (10 µl in 200 µl PCR tubes). Only functional in combination with Conductive Filtered Tips.
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Minimum computer requirements
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Desktop computer — Windows XP Pro SP2, at least 2.6 GHz Pentium 4 CPU, at least 512 MB RAM, RS-232 serial port, USB 1.1 port, DVD/CD-ROM, 80 GB free hard drive capacity, at least 1024 x 768 screen resolution; Laptop computer: Windows XP Pro SP2, at least 1.6 GHz Pentium M CPU, at least 512 MB RAM, USB 1.1 port, PCMCIA slot, DVD/CD-ROM, 80 GB free hard drive capacity, at least 1024 x 768 screen resolution
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Notebook (supplied)
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Supplied
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Pipetting head
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Single channel, 20–150 µl/s (software-controlled)
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Precision
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CV <1%, 5–200 µl (dry-well transfer); CV <5%, 2–5 µl (wet-well transfer); CV <10%, 1 µl
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Software
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Supplied with unlimited user license. Free upgrades by Web download.
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Table capacity
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6 x SBS positions (127.76 x 85.48 mm) for adapters and Tip Rack Holders; 1 x Master Mix Block; 1 x Reagent Block
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Temperature
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Operating temperature, 15–35 °C (59–95°F)
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Tip disposal
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All used tips ejected externally — no waste accumulation on the worktable
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Tip volume
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1–200 µl
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UV lamp (optional)
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UV lamp for worktable sterilization. Available only with initial purchase, in combination with the HEPA filter.
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Warranty
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1-year warranty on parts and labor
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Weight
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41 kg (90.2 lb), standard configuration
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Forensic workflow using kits from other suppliers
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Format
pdf
File size
82KB
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Download
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Forensic workflow using kits from other suppliers
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Format
pdf
File size
86KB
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Download
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Forensic workflow using kits from other suppliers
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Download
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Forensic workflow using kits from other suppliers
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Format
pdf
File size
104KB
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Download
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Forensic workflow using kits from other suppliers
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Format
pdf
File size
78KB
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Download
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Forensic workflow using kits from other suppliers
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Format
pdf
File size
78KB
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Download
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For use with QIAGEN Investigator Kits
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Format
pdf
File size
79KB
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Download
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For use with QIAGEN Investigator Kits
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Format
pdf
File size
80KB
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For use with QIAGEN Investigator Kits
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Format
pdf
File size
78KB
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For use with QIAGEN Investigator Kits
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Format
pdf
File size
101KB
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Format
pdf
File size
694KB
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Format
pdf
File size
5049KB
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Format
pdf
File size
3536KB
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Format
pdf
File size
60KB
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We strongly recommend that you update your QIAgility operating software to the new version listed above. The software update ensures optimal performance from your instrument, helps to prevent errors, and provides user-friendly features. For more information, contact QIAGEN Technical Services.
Note: QIAgility operating software is not compatible with CAS-1200 instruments. Download the latest CAS-1200 operating software.
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Format
exe
File size
23583KB
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Format
pdf
File size
407KB
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Rapid, high-precision PCR setup
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Format
pdf
File size
770KB
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Addressing critical factors and new solutions
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Format
pdf
File size
846KB
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PCR 実験における重要項目と新技術
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Format
pdf
File size
950KB
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