PyroMark Q24 Tests

For analyzing sequence variation or methylation of specific genes using the PyroMark Q24 Instrument

Detection and quantification of different mutations in a single run
Flexible software for post-run analysis of adjacent mutations
Quantification of individual or multiple CpG sites in different genes
Estimation of global CpG methylation in defined regions of interest
Sequence context provides built-in control of the assay

PyroMark Q24 Tests are a range of assays suitable for highly reliable, quantitative mutation and CpG methylation analysis and are optimized for use with the PyroMark Q24. Tests designated with 'Q24' are optimized for use with the PyroMark Q24, while others such as PyroMark HFE and PyroMark MTHFR can be used with the PyroMark Q96 ID and PyroMark Q96 MD as well. For more tests for these instruments, see PyroMark Q96 Tests.

Product	Cat. no.	Sum
PyroMark Q24 CpG LINE-1 (4 x 24) Methylation detection assay: PCR primers and sequencing primer for quantification of global methylation level in transposable elements	970042	Inquire
PyroMark Q24 CpG MGMT (4 x 24) Methylation detection assay: PCR primers and sequencing primer for quantification of methylation level in region +17 to +39 of exon 1 of the MGMT gene	970032	Inquire
PyroMark Q24 CpG MLH1 (4 x 24) Methylation detection assay: PCR primers and sequencing primer for quantification of methylation level in region –209 to –181 from the transcription start site in the MLH1 gene	970022	Inquire
PyroMark Q24 CpG p16 (4 x 24) Methylation detection assay: PCR primers and sequencing primer for quantification of methylation level in region +148 to +182 in exon 1 of the gene	970012	Inquire
PyroMark HFE (96) For detection of variants in the HFE gene (2 x 96 reactions on PyroMark Q96 MD, 1 x 96 reactions on PyroMark Q96 ID, 8 x 24 reactions on PyroMark Q24)	972442	Inquire
PyroMark MTHFR (96) For detection of variants in the MTHFR gene (2 x 96 reactions on PyroMark Q96 MD, 1 x 96 reactions on PyroMark Q96 ID, 8 x 24 reactions on PyroMark Q24)	972432	Inquire

Please log in Show cart

PyroMark Q24 Tests are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Principle

PyroMark Q24 CpG LINE-1, PyroMark Q24 CpG MGMT, PyroMark Q24 CpG MLH1, and PyroMark Q24 CpG p16 enable highly reliable methylation results. In addition, a built-in control for bisulfite treatment improves accuracy and reproducibility of the results.

Procedure

Prior to Pyrosequencing, a biotinylated PCR product is generated. Biotinylated PCR products are bound to streptavidin-coated Sepharose beads. The beads are then captured with the vacuum tool on the PyroMark Vacuum Workstation, and throroughly washed, generating single-stranded DNA suitable for sequencing. PyroMark Q24 Tests are predesigned assays for methylation analysis by Pyrosequencing. Each test includes the primers necessary for generating the biotinylated PCR product, as well as the primer for the Pyrosequencing reaction.

Applications

PyroMark Q24 Tests enable highly accurate, quantitative methylation and mutation (or SNP) analysis applicable in several fields of research, including cancer and epigenetics.

Kit Handbooks

Item actions Select all Deselect all Sort alphabetically Sort by relevance Add selection to wishlist
	PyroMark Q24 CpG LINE-1 Handbook - (EN)	Format pdf File size 265KB	Download
	PyroMark HFE Handbook - (EN)	Format pdf File size 634KB	Download
	PyroMark MTHFR Handbook - (EN)	Format pdf File size 446KB	Download

FAQs

Item actions Select all Deselect all Sort alphabetically Sort by relevance Add selection to wishlist
	2094 - How accurate and reliable is PyroMark Q24 in mutation analysis?	Format FAQ File size	View
	2856 - What is the amplicon length of the PyroMark CpG LINE-1 assay?	Format FAQ File size	View
	2857 - What region of LINE-1 gets targeted by the assay for PyroMark Q96 MD or PyroMark Q24?	Format FAQ File size	View
	2858 - How many CpG sites are analyzed by the PyroMark CpG LINE-1 assay?	Format FAQ File size	View
	2859 - Does the PyroMark CpG LINE-1 assay target mouse transposons as well?	Format FAQ File size	View
	2860 - Does the PyroMark LINE-1 assay target all LINE-1 sequences?	Format FAQ File size	View
	2861 - Can the PyroMark Q96 CpG LINE-1 assay be used with an ID system?	Format FAQ File size	View
	2940 - What should be the single peak height defined for a sample run on the different PyroMark instruments?	Format FAQ File size	View
	2827 - Can I order the nucleotides from PyroMark Gold Reagents separately?	Format FAQ File size	View
	2832 - Which purity grade is recommended for pyrosequencing primers?	Format FAQ File size	View
	2848 - How many times can vacuum troughs be re-used with the PyroMark Vacuum Preparation Stations?	Format FAQ File size	View
	2850 - Where can I order the Streptavidin Sepharose beads for pyrosequencing?	Format FAQ File size	View
	2870 - What does it mean when I get a "wide peak" error appearing at the end of a pyrosequencing run?	Format FAQ File size	View
	2871 - How many nucleotides of a homopolymer can be resolved in pyrosequencing?	Format FAQ File size	View
	2872 - Can unused wells in a pyrosequencing plate be used in the next run?	Format FAQ File size	View
	2875 - What is the reason for signals ceasing in the middle of a pyrosequencing run?	Format FAQ File size	View
	2876 - What is the reason for low peak signals in pyrosequencing?	Format FAQ File size	View
	2877 - How do I reduce background peaks in the pyrosequencing pyrogram?	Format FAQ File size	View
	2878 - How do I prevent a drifting baseline in my pyrosequencing pyrogram?	Format FAQ File size	View
	2879 - What is the reason for a high substrate peak in the pyrosequencing pyrogram?	Format FAQ File size	View
	2880 - What is the reason for peaks appearing in between dispensations on my pyrosequencing pyrogram?	Format FAQ File size	View
	2881 - What is the reason for split peaks appearing in between dispensations on my pyrosequencing pyrogram?	Format FAQ File size	View

Brochures & Guides

Item actions Select all Deselect all Sort alphabetically Sort by relevance Add selection to wishlist
	Pyrosequencing – the synergy of sequencing and quantification	Format pdf File size 2352KB	Download

Scientific Posters

Item actions Select all Deselect all Sort alphabetically Sort by relevance Add selection to wishlist
	(EN) - Pyrosequencing and its applications	Format pdf File size 2819KB	Download