Resource Center

For use with the QIAsymphony SP (software version 2.1)

Note: Please open this file only on a computer with a co-located instrument.  Failure to do so may result in an incorrect run profile.  It is recommended to review the run profile before starting a run.

The study of epigenetic mechanisms and DNA methylation has become increasingly important in many areas of research, including DNA repair, cell cycle control, developmental biology, cancer research, identification of biomarkers, predisposition factors, and potential drug targets.

Following the completion of the human genome project, the high demand for low-cost sequencing has given rise to a number of high-throughput, next-generation sequencing (NGS) technologies. These new sequencing platforms allow high-throughput sequencing for a wide range of applications.

Transfection — the delivery of DNA or RNA into eukaryotic cells — is a powerful tool used to study and control gene expression.

Useful hints for culturing animal cells (i.e., cells derived from higher eukaryotes such as mammals, birds, and insects). The guide covers different types of animal cell cultures, considerations for cell culture, and cell culture protocols.

As well as providing some general background into proteins and their biology, this guide covers commonly used protocols for expression, purification, analysis, detection and assays.

Isolation and quantification of genomic DNA from different sample sources and plasmid DNA. How to make and transform competent cells, how to culture and handle plasmid-containing cells, and commonly used techniques for analysis of genomic DNA.

This section describes considerations for isolation and quantification of RNA from different sample sources and RNA storage. It also deals with RNAi and the use of siRNA, together with miRNA, mimics, and inhibitors.

Whole genome amplification was developed as a way of increasing the amount of limited DNA samples. This is particularly useful for forensics and genetic disease research, where DNA quantities are limited, but many analyses are required. Various WGA techniques have been developed that differ both in their protocols, amplification accuracy, and ease-of-use.

This section provides a comprehensive guide to PCR. It also includes guidelines and suggestions for maximizing results from your PCR.